Gene Ontology annotation through association of InterPro records with GO terms.
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods.
Horizontal acquisition of prokaryotic hopanoid biosynthesis reorganizes membrane physiology driving lifestyle innovation in a eukaryote.
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shc1 knockout is lethal under anaerobic conditions
"S. japonicus mutant lacking shc1 was unable to grow in the absence of oxygen"
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Shc1-sfGFP localizes to ER, nuclear envelope, plasma membrane, and vacuolar membrane
"Shc1 tagged at its endogenous locus with superfolder GFP (Shc1-sfGFP) localized to cellular membranes, including the nuclear envelope (NE), cortical endoplasmic reticulum (ER) and/or the plasma membrane, and the vacuolar membranes"
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S. japonicus produces hopanoids including diplopterol and hopenes
"S. japonicus wild-type cells produced high levels of hopanoids even in the presence of oxygen, with diplopterol being the most abundant. We also detected the hopenes hop-22(29)-ene and hop-17(21)-ene"
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Hopanoids substitute for sterols during anaerobic growth
"hopanoids increased profoundly when ergosterol synthesis was inhibited either by erg1Δ mutation or the growth in anoxia"
A squalene-hopene cyclase in Schizosaccharomyces japonicus represents a eukaryotic adaptation to sterol-limited anaerobic environments.
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Functional validation showing S. japonicus produces hopanoids
"Mass spectrometry of lipid fractions of anaerobically grown Sch. japonicus showed the presence of hopanoids, a class of cyclic triterpenoids not previously detected in yeasts, including hop-22(29)-ene, hop-17(21)-ene, hop-21(22)-ene, and hopan-22-ol"
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Expression of shc1 in S. cerevisiae conferred hopanoid production and anaerobic growth
"Expression of the Sch. japonicus SHC gene (Sjshc1) in Saccharomyces cerevisiae enabled hopanoid synthesis and stimulated anaerobic growth in sterol-free media"
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Phylogenetic analysis confirmed horizontal transfer from Acetobacter
"A putative gene in Sch. japonicus showed high similarity to bacterial squalene-hopene cyclase (SHC) genes and in particular to those of Acetobacter species"
Deep research on shc1 function