BifA, a cyclic-Di-GMP phosphodiesterase, inversely regulates biofilm formation and swarming motility by Pseudomonas aeruginosa PA14.
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Purified BifA has PDE activity and no detectable DGC activity.
"purified BifA protein exhibits phosphodiesterase activity in vitro but no detectable diguanylate cyclase activity"
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Catalytic-motif mutants are stable but fail to complement the hyperbiofilm phenotype.
"In contrast, expression of either His-BifA-AAAAA or His-BifA-AAL fails to suppress the hyperbiofilm phenotype of the Δ bifA mutant."
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Deletion causes hyperbiofilm and a swarming defect.
"Deletion of the PA4367 gene results in a severe defect in swarming motility and a hyperbiofilm phenotype"
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BifA is in the inner membrane.
"BifA localizes to the inner membrane."
Binding of GTP to BifA is required for the production of Pel-dependent biofilms in Pseudomonas aeruginosa.
A genetic switch controls Pseudomonas aeruginosa surface colonization.
Induction of Native c-di-GMP Phosphodiesterases Leads to Dispersal of Pseudomonas aeruginosa Biofilms.
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Induction of native BifA or PA2133 disperses established biofilms.
"here, we demonstrate that the induction of the P. aeruginosa c-di-GMP phosphodiesterases PA2133 and BifA indeed results in the dispersal of P. aeruginosa biofilms"
Gene Ontology annotation based on Enzyme Commission mapping
Annotation inferences using phylogenetic trees