Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotation based on curation of immunofluorescence data
Quantitative Mass Spectrometry Identifies Novel Host Binding Partners for Pathogenic Escherichia coli Type III Secretion System Effectors.
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MAP7 (ensconsin) identified as host binding partner for bacterial effectors NleB1 and EspL
"we identified multiple effectors that interacted with the microtubule associated protein, ensconsin"
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Interaction occurs in region corresponding to microtubule binding domain
"we confirmed that NleB1 and EspL interacted with ensconsin in a region that corresponded to its microtubule binding domain"
A reference map of the human binary protein interactome.
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MAP7 included in HuRI protein-protein interaction dataset
"With approximately 53,000 protein-protein interactions, HuRI has approximately four times as many such interactions as there are high-quality curated interactions from small-scale studies"
Identification and molecular characterization of E-MAP-115, a novel microtubule-associated protein predominantly expressed in epithelial cells.
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Original characterization of MAP7/E-MAP-115/Ensconsin
"A novel microtubule-associated protein (MAP) of M(r) 115,000 has been identified"
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Demonstrated microtubule binding via N-terminal alpha-helical domain
"A novel microtubule-binding site has been localized to the basic alpha-helical region in the NH2-terminal domain"
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Showed microtubule stabilization activity (nocodazole resistance)
"Overexpression of this domain of E-MAP-115 by transfection of fibroblasts lacking significant levels of this protein with its cDNA renders microtubules stable to nocodazole"
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Expression increases during epithelial cell polarization
"In Caco-2 cells, labeling for E-MAP-115 increases when they polarize and form blisters"
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Predominantly expressed in epithelial cells
"E-MAP-115 is a microtubule-stabilizing protein that may play an important role during reorganization of microtubules during polarization and differentiation of epithelial cells"
Competition between microtubule-associated proteins directs motor transport
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MAP7 promotes kinesin-1 landing and activity (~15-fold increase in landing rate)
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MAP7 inhibits kinesin-3
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MAP7 displaces tau from microtubules (higher affinity, longer dwell time)
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Quantified binding parameters for MAP7-MT interaction
A structural and dynamic visualization of the interaction between MAP7 and microtubules
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MTBD is primarily alpha-helical with a short hinge
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MTBD binds across more than one tubulin dimer
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Interaction involves tubulin C-terminal tails
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KD ~0.94 uM for MTBD-MT binding by ITC
Map7/7D1 and Dvl form a feedback loop that facilitates microtubule remodeling and Wnt5a signaling
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MAP7 binds Dishevelled (Dvl)
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MAP7-KIF5B complex mediates plus-end MT targeting
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Role in Wnt5a signaling and epithelial polarity
Deep research summary for MAP7 gene function
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MAP7 is a structural regulator/adaptor on MTs that stabilizes MTs and recruits kinesin-1
"MAP7 is a structural regulator/adaptor on MTs that both stabilizes/bundles MTs and recruits/activates kinesin-1 to the lattice to promote plus-end-directed transport."
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Quantified motor recruitment metrics from single-molecule studies
"MAP7 increases K560 (kinesin-1) landing rate ~15-fold; lowers KmMT (1.46 +/- 0.21 uM to 0.27 +/- 0.04 uM); slightly increases processivity (~874 to ~984 nm) and decreases velocity (~434 to ~328 nm/s)."
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Structural basis of MT binding via MTBD
"MAP7 MTBD-MT binding KD ~0.94 uM (ITC) with a stoichiometry of ~0.5 per tubulin tetramer context; binding extends beyond a single tubulin dimer and involves tubulin C-terminal tails."
Cyberian deep research on MAP7 function