PA2870 (Q9HZX6) review notes

Summary of the literature

PA2870 is a 525-aa protein with two predicted TM helices (UniProt Phobius, 20-40
and 184-207) flanking a periplasmic region, and a C-terminal GGDEF domain (391-525).
My scan of the UniProt sequence finds the GGEEF motif at 440. No EAL domain.
No name has been assigned.

Literature search (Europe PMC, 2026-11-05, mostly 503 errors; PubMed E-utilities
500 errors). The gene is mentioned only in genome-wide c-di-GMP surveys:

  1. Kulasekara et al. 2006 PMID:16477007; full text read on PMC (PMC1413825).
  2. Table 1: "PA2771 GGEEF — PA2818 EAL — PA2870 GGEEF +(96)" (pmol c-di-GMP per mg
    wet cell weight in extracts of overexpressing PA14; detection limit 12).
  3. "P. aeruginosa expressing PA2870 and PA3343, two DGCs that produce high levels
    of c-di-GMP in our assay ( Table 1 ), do not cause an alteration in the biofilm
    phenotype."
  4. Merritt et al. 2010 PMID:20978535: a single-crossover PA2870 mutant had no
    consistent biofilm defect PMID:20978535.
  5. Eilers et al. 2022 PMID:35935233: the PAO1 deletion reduced early attachment
    PMID:35935233.

PseudoCAP IBA-seed assessment

PTN node node term n_seeds leaf IBAs seed annotation (term, evidence, PMID) verdict rationale other seeds IBD stands without this seed?
PTN002895344 GO:0052621 diguanylate cyclase activity 19 205 (28 taxa) GO:0052621, IDA, PMID:16477007 (PseudoCAP) SOUND Extracts of the overexpressing strain contained 96 pmol c-di-GMP/mg (full text read); GGEEF motif intact. Crude whole-cell assay, no purified protein. E. coli DosC, DgcC/J/M/N/P/Q/Z; B. subtilis DgcK, DgcP; Shewanella DgcS; P. aeruginosa WspR, SadC, GcbA, DgcP, PA3177, PA1107, PA3343 Yes; see PA1107 notes. No GO_CENTRAL defect for the MF.

PTN002895351 (GO:0043709 IBD): the node-level GO_CENTRAL defect is recorded once in
PA1107-ai-review.yaml (see PA1107 notes); not repeated here.

Annotation decisions