Gene Ontology annotation through association of InterPro records with GO terms

Annotation inferences using phylogenetic trees

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

GO_REF:0000052

Gene Ontology annotation based on curation of immunofluorescence data

GO_REF:0000117

Electronic Gene Ontology annotations created by ARBA machine learning models

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

PMID:10942414

Human erythrocyte pyrimidine 5-nucleotidase, PN-I, is identical to p36, a protein associated to lupus inclusion formation in response to alpha-interferon.

Recombinant PN-I is a fully active pyrimidine 5'-nucleotidase and is identical to p36, the interferon-alpha-induced protein.

PMID:11795870

Human erythrocyte pyrimidine 5'-nucleotidase, PN-I.

Purified/recombinant PN-I is a monomeric ~36 kDa, Mg2+-dependent enzyme specific for pyrimidine nucleoside monophosphates, with an associated pyrimidine phosphotransferase activity.

PMID:12930399

Molecular characterization of six unrelated Italian patients affected by pyrimidine 5'-nucleotidase deficiency.

P5ND is an autosomal recessive hemolytic anemia with basophilic stippling and intra-erythrocyte accumulation of pyrimidine nucleotides; several loss-of-function NT5C3A variants identified.

PMID:15968458

Evidence for essential catalytic determinants for human erythrocyte pyrimidine 5'-nucleotidase.

Recombinant PN-I dephosphorylates pyrimidine nucleoside monophosphates; site-directed mutagenesis defines essential catalytic residues (e.g. Asp88, Asp90, Glu135).

PMID:21478870

A diverse range of gene products are effectors of the type I interferon antiviral response.

NT5C3A was included in a large ISG antiviral overexpression screen but behaved as a weak/non-effector (post-infection effect on HCV replication less than 20%).

PMID:32296183

A reference map of the human binary protein interactome.

Systematic yeast two-hybrid map (HuRI) reporting NT5C3A binary interactions (e.g. DESI1, UBQLN2); source of the bare "protein binding" IPI annotations.

PMID:34800366

Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.

High-throughput mitochondrial proteome dataset in which NT5C3A was detected; basis for the HTP mitochondrion location annotation.

PMID:8557639

Purification, microsequencing, and immunolocalization of p36, a new interferon-alpha-induced protein that is associated with human lupus inclusions.

p36 (= NT5C3A isoform 2) is an interferon-alpha-induced protein that localizes to the endoplasmic reticulum, co-localizing with protein disulfide isomerase and lupus inclusions.

PMID:9428647

Pyrimidine nucleotidases from human erythrocyte possess phosphotransferase activities specific for pyrimidine nucleotides.

Erythrocyte pyrimidine nucleotidases (PN-I/PN-II) preferentially hydrolyse pyrimidine 5'- (or 3'-) monophosphates and possess a pyrimidine-specific phosphotransferase activity; no tRNA 2'-phosphotransferase activity is described.

Reactome:R-HSA-109449

(d)CMP, TMP, or (d)UMP + H2O => (deoxy)cytidine, thymidine, or (deoxy)uridine + orthophosphate (NT5C3)

Reactome reaction placing NT5C3(A) in the cytosol, hydrolyzing pyrimidine (deoxy)nucleoside monophosphates to nucleoside plus orthophosphate.