Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Distribution of CTP:phosphocholine cytidylyltransferase (CCT) isoforms. Identification of a new CCTbeta splice variant.
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CCTβ1 and CCTβ2 both localize to the endoplasmic reticulum, in contrast to CCTα which is also nuclear; CCTβ2 has in vitro enzymatic activity and complements the temperature-sensitive cytidylyltransferase defect in CHO58 cells.
"both CCTbeta1 and CCTbeta2 localized to the endoplasmic reticulum of cells, in contrast to CCTalpha which resided in the nucleus in addition to associating with the endoplasmic reticulum."
Cloning and characterization of a second human CTP:phosphocholine cytidylyltransferase.
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Cloning of CCTβ, a second human CCT distinct from CCTα; it requires lipid regulators for maximal activity, localizes to the cytoplasm, and its overexpression drives CCT activity, CDP-choline accumulation, and phosphatidylcholine synthesis.
"Transfection of COS-7 cells with a CCTbeta expression construct led to the overexpression of CCT activity, the accumulation of cellular CDP-choline, and enhanced radiolabeling of phosphatidylcholine."
PCho and CTP are condensed to CDP-Cho by PCYT1 dimer
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At the ER membrane, the active membrane-bound PCYT1A or PCYT1B homodimer condenses phosphocholine and CTP to produce CDP-choline.
"At the endoplasmic reticulum (ER) membrane, active membrane-bound choline-phosphate cytidylyltransferase A (PCYT1A) or B (PCYT1B) homodimer condenses phosphocholine (PCho) and cytidine triphosphate (CTP) to produce CDP-choline (CDP-Cho)"