Gene Ontology annotation through association of InterPro records with GO terms
Malonic semialdehyde reductase, succinic semialdehyde reductase, and succinyl-coenzyme A reductase from Metallosphaera sedula: enzymes of the autotrophic 3-hydroxypropionate/4-hydroxybutyrate cycle in Sulfolobales.
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Msed_1424 is the succinic semialdehyde reductase, identified by purification from autotrophic M. sedula and peptide mass fingerprinting.
"The best hit was with the gene gi|146304190 (100% identity, 65% coverage) annotated as a Zn-dependent alcohol dehydrogenase gene encoding a 37.9-kDa protein (Msed_1424; 360 amino acids)."
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The recombinant enzyme is a highly active, homodimeric, NADPH-preferring reductase.
"The purified enzyme catalyzed the expected reaction at a rate of 700 μmol min −1 mg −1 protein at 65°C."
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The enzyme contains about two zinc ions per monomer.
"Metal analysis (27 elements) of succinic semialdehyde reductase using plasma emission spectroscopy revealed the presence of 1.9 mol of zinc per mol of enzyme monomer, whereas other metals could not be detected."
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The succinyl-CoA reductase step is catalysed by Msed_0709, not by Msed_1424.
"Interestingly, succinyl-CoA reductase turned out to be identical to malonyl-CoA reductase."
A 3-hydroxypropionate/4-hydroxybutyrate autotrophic carbon dioxide assimilation pathway in Archaea.
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Defines the 3HP/4HB cycle, in which succinyl-CoA is reduced to 4-hydroxybutyrate.
"This intermediate was reduced to 4-hydroxybutyrate and converted into two acetyl-CoA molecules via 4-hydroxybutyryl-CoA dehydratase."
The genome sequence of the metal-mobilizing, extremely thermoacidophilic archaeon Metallosphaera sedula provides insights into bioleaching-associated metabolism.
Identification of missing genes and enzymes for autotrophic carbon fixation in crenarchaeota.
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The Thermoproteus neutrophilus homolog of Msed_1424 (Tneu_0419) is also a succinic semialdehyde reductase of the alcohol dehydrogenase family.
"T. neutrophilus contains a gene in the autotrophy-related gene cluster that is similar (53 and 75% aa identity and similarity) to M. sedula succinic semialdehyde reductase (Msed_1424)"
Reaction kinetic analysis of the 3-hydroxypropionate/4-hydroxybutyrate CO(2) fixation cycle in extremely thermoacidophilic archaea.
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Msed_1424 was used as the succinic semialdehyde reductase (ssr) in the kinetic reconstruction of the cycle.
"The genes hpcs (Msed_1456), hpcd (Msed_2001), acr (Msed_1426), mcr (Msed_0709), and ssr (Msed_1424) were amplified from genomic DNA using the primers listed in Supplementary Table S1."
UniProt record A4YGN0 (SUCD_METS5)