Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Systematic multi-level analysis of an organelle proteome reveals new peroxisomal functions.
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Ykl050c was identified as a peroxisomal matrix protein in a systematic multi-level analysis of the yeast peroxisome proteome, and named Lpx2 (Lipase of Peroxisomes 2).
"an additional newly identified peroxisomal lipase, hence we named Ykl050c, Lpx2 (Lipase of Peroxisomes 2)"
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Deletion of YKL050C caused the most significant change in the yeast lipidome in glucose medium, an increase in lyso-phosphatidylglycerol (LPG), consistent with a role in glycerophospholipid metabolism.
"Δykl050c shows the most significant change in glucose conditions, with an increase of LPG lipids."
The yeast peroxisomal proteome at absolute quantitative scale.
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Lpx2/YKL050C was detected in a 2026 absolute-quantification study of yeast peroxisomal proteins and was one of the peroxisomal proteins whose abundance was higher under glucose than oleate growth, consistent with its partial rather than exclusive peroxisomal localization.
"These include the enzymes Tsr3, Pre2, Glr1, and Lpx2 (Fig. 2d), which only partially localize to peroxisomes (Yifrach et al. 2022)"
Screening for hydrolytic enzymes reveals Ayr1p as a novel triacylglycerol lipase in Saccharomyces cerevisiae.
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A functional-proteomics screen of hydrolase candidates carrying the GXSXG lipase motif on peroxisomes and lipid droplets. Ykl050cp (LPX2) was one of the screened GXSXG-motif candidates, and overexpression analysis proposed a role for it in TG mobilization; but in the definitive in-vivo (and in-vitro) lipolytic assays only Lpx1p and Ayr1p were confirmed as lipases, whereas all other candidates (Ykl050cp included) did NOT exhibit lipolytic activity in vivo.
"In vivo mobilization of TG by Lpx1p and Ayr1p confirmed the role of these two proteins as lipases in living cells, whereas all other enzymes did not exhibit lipolytic activities in vivo"
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Ykl050cp is explicitly named among the short list of putative novel TG lipases carrying the classical GXSXG lipase motif, confirming that this study did assay Ykl050c rather than only its paralogs.
"The short list of putative novel TG lipases included Lpx1p, Ldh1p, Yju3p, Ayr1p, Eht1p, Tsc10p, Ybr056wp, and Ykl050cp."
The function and properties of the Azf1 transcriptional regulator change with growth conditions in Saccharomyces cerevisiae.
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LPX2/YKL050C expression is regulated by the Azf1 transcription factor, which activates carbon and energy metabolism genes in glucose.
"in glucose, Azf1 activates"
Genome-wide surveys for phosphorylation-dependent substrates of SCF ubiquitin ligases.
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LPX2/YKL050C is reported by SGD as a substrate of the SCF(Cdc4) ubiquitin ligase complex, identified in a genome-wide phospho-dependent-substrate survey.
"phosphorylation-dependent substrates of SCF ubiquitin ligases"
SGD gene description for LPX2 (yeast)