Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Expression cloning of human globoside synthase cDNAs. Identification of beta 3Gal-T3 as UDP-N-acetylgalactosamine:globotriaosylceramide beta 1,3-N-acetylgalactosaminyltransferase.
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Expression cloning and substrate-specificity analysis identified B3GALNT1 (beta3Gal-T3) as the human globoside synthase that specifically transfers N-acetylgalactosamine onto globotriaosylceramide (Gb3), producing globoside; requires Mg2+ and is expressed in heart, brain, testis and other tissues.
"specifically catalyzes the transfer of N-acetylgalactosamine onto"
B3GALNT1 transfers GalNAc to Gb3Cer to form Gb4Cer
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B3GALNT1, a Golgi membrane-associated protein, adds N-acetylgalactosamine to globotriaosylceramide (Gb3Cer) to form Gb4Cer.
"UDP-GalNAc:beta-1,3-N-acetylgalactosaminyltransferase 1 (B3GALNT1, B3GALT3) is a Golgi membrane-associated protein that can add N-acetylgalactosamine (GalNAc) to globotriaosylceramide (Gb3Cer) to form Gb4Cer"
Glycosphingolipid biosynthesis
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Parent pathway describing synthesis of glycosphingolipids, within which B3GALNT1 performs the globo-series Gb3Cer -> Gb4Cer step.
"The steps involved in the synthesis of glycosphingolipids (sphingolipids with one or more sugars attached) are annotated here"