Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotation based on curation of immunofluorescence data
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Automatic Gene Ontology annotation based on Rhea mapping
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Role of human sphingosine-1-phosphate phosphatase 1 in the regulation of intra- and extracellular sphingosine-1-phosphate levels and cell viability.
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Human SPPase1 (SGPP1) dephosphorylates S1P and dihydro-S1P; overexpression raises S1P-directed phosphatase activity and knockdown raises cellular and secreted S1P with a fall in sphingosine, establishing the core catalytic function and its role in setting S1P levels and cell viability.
"Transient overexpression of hSPPase1 exhibited a 2-fold increase in phosphatase activity against S1P and dihydro-S1P"
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The human enzyme localizes to the endoplasmic reticulum, shown by confocal co-localization with calreticulin.
"co-localized to endoplasmic reticulum with calreticulin"
Sphingosine-1-phosphate phosphohydrolase regulates endoplasmic reticulum-to-golgi trafficking of ceramide.
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SPP-1 (SGPP1) regulates ER ceramide levels and thereby influences anterograde ER-to-Golgi transport of both ceramide and proteins; substrate specificity is restricted to sphingoid-1-phosphates (not ceramide-1-phosphate, LPA or PA).
"SPP-1 regulates ceramide levels in the ER and thus influences the anterograde membrane transport of both ceramide and proteins from the ER to the Golgi apparatus"
SGPP1,2 dephosphorylate sphingoid-1-phosphates