Annotation inferences using phylogenetic trees

Gene Ontology annotation based on UniPathway vocabulary mapping

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

Combined Automated Annotation using Multiple IEA Methods

Global analysis of protein localization in budding yeast.

Chemogenomic approach identified yeast YLR143W as diphthamide synthetase.

The amidation step of diphthamide biosynthesis in yeast requires DPH6, a gene identified through mining the DPH1-DPH5 interaction network.