GO_REF:0000033

Annotation inferences using phylogenetic trees

Provides phylogenetic support for cytoplasmic localization and uridine kinase activity

GO_REF:0000043

Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping

Automated annotations based on UniProtKB keywords for nucleotide binding and transferase activity

GO_REF:0000108

Automatic assignment of GO terms using logical inference, based on on inter-ontology links.

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods.

Automated annotations combining multiple computational methods for molecular functions and biological processes

PMID:11306702

Phosphorylation of uridine and cytidine nucleoside analogs by two human uridine-cytidine kinases.

UCK1 phosphorylates uridine and cytidine to UMP and CMP respectively

"The approximately 30-kDa proteins, named UCK1 and UCK2, were expressed in Escherichia coli and shown to catalyze the phosphorylation of Urd and Cyd."
Does not phosphorylate deoxyribonucleosides or purine ribonucleosides

"The enzymes did not phosphorylate deoxyribonucleosides or purine ribonucleosides."
Can use ATP or GTP as phosphate donor

"Uridine-cytidine kinases (UCK) have important roles for the phosphorylation of nucleoside analogs that are being investigated for possible use in chemotherapy of cancer."
Phosphorylates various nucleoside analogs including 6-azauridine, 5-fluorouridine, 4-thiouridine, 5-bromouridine, N(4)-acetylcytidine, N(4)-benzoylcytidine, 5-fluorocytidine, 2-thiocytidine, 5-methylcytidine, and N(4)-anisoylcytidine

"The enzymes phosphorylated several of the analogs, such as 6-azauridine, 5-fluorouridine, 4-thiouridine, 5-bromouridine, N(4)-acetylcytidine, N(4)-benzoylcytidine, 5-fluorocytidine, 2-thiocytidine, 5-methylcytidine, and N(4)-anisoylcytidine."
UCK1 mRNA detected in multiple tissues with ubiquitous expression

"The 2.7-kb band was ubiquitously expressed in the investigated tissues."

Reactome:R-HSA-73599

cytidine or uridine + ATP => CMP or UMP + ADP [UCK1]

UCK1 localizes to the cytosol

"Cytosolic uridine-cytidine kinase 1 (UCK1) catalyzes the reactions of cytidine or uridine with ATP to form CMP or UMP and ADP"
Catalyzes phosphorylation of cytidine or uridine with ATP to form CMP or UMP and ADP

"Cytosolic uridine-cytidine kinase 1 (UCK1) catalyzes the reactions of cytidine or uridine with ATP to form CMP or UMP and ADP"
Functions as a tetramer based on crystallographic data

"Unpublished crystallographic data show the enzyme to be a tetramer (PDB - 2JEO)."

file:human/UCK1/UCK1-deep-research-perplexity-lite.md

Deep research on UCK1 function

PMID:27239701

The pivotal role of uridine-cytidine kinases in pyrimidine metabolism and activation of cytotoxic nucleoside analogues in neuroblastoma.

UCK1-GFP localized to the nucleus, while UCK2-GFP localized to the cytosol, and UCK1 co-expression redirected UCK2 to the nucleus.

"Subcellular localization studies showed that UCK1-GFP and UCK2-GFP were localized in the cell nucleus and cytosol, respectively."

file:human/UCK1/UCK1-deep-research-falcon.md

Falcon deep research on UCK1 function

file:human/UCK1/UCK1-uniprot.txt

UniProt record for human UCK1