MSLN curation notes

Deep research (falcon/perplexity-lite) unavailable; notes from UniProt Q13421 and cached papers.

• Precursor (622 aa) -> furin cleavage -> secreted MPF (37-286) + GPI-anchored mesothelin (296-598)
  (UniProt PTM, PMID:8552591).
• Surface/GPI: PMID:8552591.
• CA125/MUC16 binding mediates heterotypic adhesion PMID:14676194.
• MPF secreted, elevated in mesothelioma serum (PMID:16857795); MPF activity (PMID:7665620).
• N-terminal fragment structure with MORAb-009 overlapping CA125 footprint (PMID:22787150).

Localization conclusion: PM outer leaflet (GPI-anchored), mature ectodomain fully extracellular and
surface-accessible; shed/soluble and MPF pools extracellular; ER/Golgi only in transit.