Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on UniPathway vocabulary mapping
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Automatic Gene Ontology annotation based on Rhea mapping
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Identification and characterization of HAOX1, HAOX2, and HAOX3, three human peroxisomal 2-hydroxy acid oxidases.
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HAOX1 (HAO1) is a peroxisomal 2-hydroxy acid oxidase most active on glycolate, also active on 2-hydroxy fatty acids, and able to oxidize glyoxylate to oxalate, implicating it in primary hyperoxaluria type 1 pathophysiology. Expressed primarily in liver and pancreas.
"substrate, glycolate, but is also active on 2-hydroxy fatty acids"
Purification and characterization of recombinant human liver glycolate oxidase.
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Recombinant human liver glycolate oxidase is an FMN-dependent peroxisomal oxidase belonging to the L-2-hydroxy-acid-oxidizing flavoenzyme family; it contributes to oxalate production and is inhibited by its product oxalate.
"an FMN-dependent peroxisomal oxidase"
Active site and loop 4 movements within human glycolate oxidase: implications for substrate specificity and drug design.
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Crystal structures and kinetics of human glycolate oxidase (HAO1) show that oxidation of glycolate to glyoxylate is the primary reaction, that glyoxylate-to-oxalate oxidation is likely not physiologically relevant, and that the enzyme is a drug target for primary hyperoxaluria type 1.
"oxidation of glycolate to glyoxylate is the primary reaction catalyzed by GO, while the oxidation of glyoxylate to oxalate is most likely not relevant under normal conditions"
HAO1 tetramer oxidizes glycolate to glyoxylate
Conversion of glyoxylate to oxalate
Cargo of PEX5S,L translocates from the cytosol to the peroxisomal matrix
PEX5S,L:Cargo binds PEX13:PEX14:PEX2:PEX10:PEX12 (Docking and Translocation Module)
UniProtKB Q9UJM8 (HAOX1_HUMAN) 2-Hydroxyacid oxidase 1
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HAO1 is a broad-specificity (S)-2-hydroxy-acid oxidase that preferentially oxidizes glycolate, uses O2 to produce H2O2, is an FMN-dependent homotetramer in the peroxisome matrix, is highly expressed in liver, and feeds glyoxylate into peroxisomal glycine biosynthesis.
"preferentially oxidizes glycolate"