Gene Ontology annotation through association of InterPro records with GO terms
TreeGrafter-generated GO annotations
Combined Automated Annotation using Multiple IEA Methods
UniProtKB entry Q88HG4 for Pseudomonas putida KT2440 PP_3394
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The fetched UniProt record contains an alpha-isopropylmalate/homocitrate-synthase signature, in conflict with its electronic HMG-CoA lyase assignment.
"InterPro; IPR002034; AIPM/Hcit_synth_CS."
The Pseudomonas aeruginosa liuE gene encodes the 3-hydroxy-3-methylglutaryl coenzyme A lyase, involved in leucine and acyclic terpene catabolism.
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Biochemical characterization of P. aeruginosa LiuE provides the positive HMG-CoA lyase comparator against which the conflicting PP_3394 signatures were evaluated.
"cleavage of HMG-CoA to acetyl-CoA and acetoacetate."
OpenScientist gene research for PSEPK mvaB
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The provider reports that MvaB is much closer to characterized P. aeruginosa LiuE than it is to PP_3394.
"| mvaB (PP_3540) vs. *P. aeruginosa* LiuE/PA2011 (characterized HMG-CoA lyase) | **78.6%** |"
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The provider reports moderate identity between the two KT2440 paralogs; it does not report a direct PP_3394-to-LiuE comparison.
"| mvaB vs. KT2440 paralog PP_3394 | 41.8% |"
Cached OpenScientist BCAA-catabolism module report for Pseudomonas putida KT2440
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The generated module report explicitly leaves PP_3394 versus MvaB unresolved as the displaced HMG-CoA lyase candidate.
"Both are candidates for the displaced leucine terminal step; choice is **unresolved** and should be flagged."