Gene Ontology annotation through association of InterPro records with GO terms
Combined Automated Annotation using Multiple IEA Methods
UniProtKB entry C5CY05 (UREG_VARPS): Urease accessory protein UreG
-
Vapar_4248 (C5CY05, ureG) is a Swiss-Prot reviewed 216-residue cytoplasmic SIMIBI-class G3E GTPase (HAMAP rule MF_01389, InterPro IPR004400, PANTHER PTHR31715:SF0) with a conserved GTP-binding motif at residues 24-31 that facilitates GTP-hydrolysis-dependent incorporation of the urease nickel metallocenter into UreC.
"CC -!- FUNCTION: Facilitates the functional incorporation of the urease nickel CC metallocenter. This process requires GTP hydrolysis, probably CC effectuated by UreG. {ECO:0000255|HAMAP-Rule:MF_01389}."
-
Vapar_4248 binds GTP at its conserved P-loop nucleotide-binding motif (residues 24-31).
"FT BINDING 24..31 FT /ligand="GTP" FT /ligand_id="ChEBI:CHEBI:37565" FT /evidence="ECO:0000255|HAMAP-Rule:MF_01389""
GTP-dependent activation of urease apoprotein in complex with the UreD, UreF, and UreG accessory proteins.
-
Activation of urease apoprotein in the UreD-UreF-UreG complex at physiological bicarbonate concentrations requires GTP hydrolysis catalyzed at the nucleotide-binding site of UreG.
"The critical nucleotide-binding site was localized to UreG on the basis of experiments using a variant complex."
Klebsiella aerogenes UreF: identification of the UreG binding site and role in enhancing the fidelity of urease activation.
-
Klebsiella aerogenes UreG directly binds one nickel or zinc ion with micromolar affinity and serves as the GTPase in the UreD-UreF-UreG activation complex.
"In K. aerogenes this protein is a monomer that binds one Ni or one Zn with Kd values of about 5 μM"
Complete genome sequence of the metabolically versatile plant growth-promoting endophyte Variovorax paradoxus S110.
-
Genomic analysis of Variovorax paradoxus S110 highlights its diverse metabolic capabilities in soil and plant-associated environments.
"Variovorax paradoxus is a microorganism of special interest due to its diverse metabolic capabilities, including the biodegradation of both biogenic compounds and anthropogenic contaminants."