Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
The BBSome controls IFT assembly and turnaround in cilia.
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The BBSome assembles IFT particles at the ciliary base and regulates IFT turnaround/recycling at the ciliary tip.
"the in vivo function for the BBSome is to regulate the assembly of the IFT particles at the ciliary base"
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The BBSome binds the IFT particle as a cargo, not as an integral structural component.
"the BBSome binds to the IFT particle like a cargo but not a structural component"
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BBS-4 loses ciliary localization and accumulates at the ciliary base when the BBSome is uncoupled from moving IFT.
"Some of them (BBS-1, BBS-4) totally lost the ciliary localization"
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The BBSome shares coat features with COPI/COPII/clathrin and can directly recognize IFT cargo.
"shares the common structural features with COPI, COPII, and clathrin coats, and can directly recognize IFT cargos"
BBS4 and BBS5 show functional redundancy in the BBSome to regulate the degradative sorting of ciliary sensory receptors.
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BBS-4 directly interacts with BBS-5 through its C-terminal region (a.a. 270-462).
"mapped down the binding region in BBS-4 to the C-terminal 193 amino acids (a.a. 270–462)"
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bbs-4 and bbs-5 single mutants have normal cilia; bbs-4; bbs-5 double mutants phenocopy whole-BBSome loss.
"bbs-4 or bbs-5 single mutants are completely normal in dye-filling assay"
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BBS-4 and BBS-5 redundantly regulate lysosome-targeted degradation of ciliary sensory receptors.
"BBS-4 and BBS-5 play redundant role in the BBSome to ubiquitously regulate the lysosome-targeted degradation of ciliary sensory receptors"
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The BBSome acts upstream of the early endosome at the ciliary base to direct receptors to lysosomal degradation.
"the BBSome acts upstream of the early endosome, probably in endocytic stage at cilia base, to regulate the lysosomal sorting of ciliary receptors"
Deep research report on bbs-4 (Edison/falcon)