Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Combined Automated Annotation using Multiple IEA Methods
A novel class of microbial phosphocholine-specific phospholipases C.
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The 1,500-fold purified PlcHR2 heterodimer hydrolyses only choline-containing phospholipids, phosphatidylcholine and sphingomyelin equally.
"PlcHR2 is only active on choline-containing phospholipids. It is equally active on phosphatidylcholine (PC) and sphingomyelin (SM) and is able to hydrolyse plasmenylcholine phospholipids (plasmalogens)"
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Purified PlcHR2 is haemolytic, and calcium inhibits the haemolytic activity without affecting the enzymatic activity.
"While calcium has no detectable effect on enzymatic activity, it inhibits the haemolytic activity of PlcHR2"
Molecular comparison of a nonhemolytic and a hemolytic phospholipase C from Pseudomonas aeruginosa.
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PLC-H hydrolyses sphingomyelin as well as phosphatidylcholine, unlike PLC-N, which also acts on phosphatidylserine.
"PLC-H hydrolyzes sphingomyelin in addition to phosphatidylcholine"
Purification, characterization, and identification of a sphingomyelin synthase from Pseudomonas aeruginosa. PlcH is a multifunctional enzyme.
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Purified PlcH has sphingomyelin synthase activity, and the activity is absent from plcH operon knockouts.
"Second, purified PlcH showed SM synthase activity"
Involvement of the twin-arginine translocation system in protein secretion via the type II pathway.
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Two P. aeruginosa phospholipases, including PlcH, use the Tat system for inner-membrane translocation before the Xcp secreton.
"two substrates for the Pseudomonas aeruginosa secreton, both phospholipases, use the twin-arginine translocation (Tat) system"
Anr and its activation by PlcH activity in Pseudomonas aeruginosa host colonization and virulence.
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PlcH degrades host phosphatidylcholine; the choline and glycine betaine released activate the Anr regulon, and a plcHR mutant is defective in colonization and biofilm formation.
"A ΔplcHR mutant, known to be defective in virulence in animal models, was less able to colonize epithelial cell monolayers and was defective in biofilm formation on plastic when grown in lung surfactant"