GO_REF:0000024

Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity

GO_REF:0000033

Annotation inferences using phylogenetic trees

GO_REF:0000044

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

GO_REF:0000107

Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara

GO_REF:0000117

Electronic Gene Ontology annotations created by ARBA machine learning models

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

PMID:29449551

Mutations in CFAP43 and CFAP44 cause male infertility and flagellum defects in Trypanosoma and human.

A CFAP43-mutated human patient has profound sperm axoneme disorganization, including central-pair loss; mouse disruption corroborates the flagellar architectural requirement.

"the absence of the CPC"
Endogenous tagged Trypanosoma CFAP43 and CFAP44 localize along the axoneme. Their doublet-5/6 positioning relative to a paraflagellar rod is not automatically transferred to human cilia.

PMID:31884020

CFAP43 modulates ciliary beating in mouse and Xenopus.

Mouse Cfap43 loss increases tracheal beat frequency while decreasing bead transport. Gross respiratory-cilium structure can remain intact, separating beat regulation from sperm architectural requirements.

PMID:29514928

The I1 dynein-associated tether and tether head complex is a conserved regulator of ciliary motility.

Tetrahymena fap43 knockout removes the tether/tether-head structure while averaged I1 dynein and other axonemal components appear retained.

"both the tether and tether head structures were missing"
Tagged termini and structural analyses support FAP43/FAP44 complex organization; they do not demonstrate that human CFAP43 is an ATPase or universally required for inner-arm assembly.

PMID:29687140

Ciliary proteins Fap43 and Fap44 interact with each other and are essential for proper cilia and flagella beating.

Tetrahymena Fap43 localizes along cilia; knockout preserves cilia number/length but changes their waveform and reduces swimming.

"the waveform of the cilia beat was altered"
Native-promoter tagged ciliary protein recovery and proximity experiments connect Fap43 with Fap44 and nearby axonemal machinery, without implying every association is a purified direct interface.

PMID:37258679

Axonemal structures reveal mechanoregulatory and disease mechanisms.

Algal FAP43 and FAP44 form the tether/tether-head apparatus; human respiratory-axoneme reconstruction identifies the conserved machinery and canonical-sequence CFAP43.

"a coiled coil with FAP43"

PMID:39743588

Structural diversity of axonemes across mammalian motile cilia.

CFAP43/CFAP44 helices participate in the inner-arm docking network; the bovine prestroke structure adds context for the complex’s mechanical role.

"C-terminal helices of CFAP44 and CFAP43"

PMID:28552195

Biallelic Mutations in CFAP43 and CFAP44 Cause Male Infertility with Multiple Morphological Abnormalities of the Sperm Flagella.

Human variant and mouse knockout findings corroborate the conserved male-infertility and sperm-flagellar phenotype.

PMID:31004071

Nonsense mutation in CFAP43 causes normal-pressure hydrocephalus with ciliary abnormalities.

A family with a CFAP43 truncating variant and a separate mouse disruption study connect ciliary abnormalities with hydrocephalus context, without defining a new molecular activity.