Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotation based on curation of immunofluorescence data
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Automatic Gene Ontology annotation based on Rhea mapping
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Changes in levels of normal ML-1 gene transcripts associated with the conversion of human nontumorigenic to tumorigenic phenotypes.
Heteromeric interactions required for abundance and subcellular localization of human CDC50 proteins and class 1 P4-ATPases.
Human Type IV P-type ATPases That Work as Plasma Membrane Phospholipid Flippases and Their Regulation by Caspase and Calcium.
Expression and functional characterization of missense mutations in ATP8A2 linked to severe neurological disorders.
Genetic and age related models of neurodegeneration in mice: dystrophic axons.
Critical role of the beta-subunit CDC50A in the stable expression, assembly, subcellular localization, and lipid transport activity of the P4-ATPase ATP8A2.
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The bovine ATP8A2-CDC50A complex transports PS more efficiently than PE; the construct species is established in the separately inspected Methods.
"actively transports phosphatidylserine and to a lesser extent
phosphatidylethanolamine across membranes."
P4-ATPase ATP8A2 acts in synergy with CDC50A to enhance neurite outgrowth.
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ATP8A2 overexpression increased neurite extension in PC12 and primary rat neuronal cultures; the abstract does not establish the expressed construct species.
"Overexpression of ATP8A2 increased the length of neurite
outgrowth in NGF-induced PC12 cells and in primary cultures of rat hippocampal
neurons."
Mutations in a P-type ATPase gene cause axonal degeneration.
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The mouse ATP8A2 study supports direct inward PS translocation, consistent with its separately inspected construct and uptake experiments.
"ATP8A2 possesses phosphatidylserine translocase activity and is involved in
localization of phosphatidylserine to the inner leaflet of the plasma membrane."
Phospholipid flippase ATP8A2 is required for normal visual and auditory function and photoreceptor and spiral ganglion cell survival.
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ATP8A2 loss in mice impaired photoreceptor structure, responses and survival; this endpoint does not itself distinguish early lamination from later degeneration.
"ATP8A2-deficient mice have shortened photoreceptor outer segments, a reduction
in photoresponses and decreased photoreceptor viability."
A sublethal ATP11A mutation associated with neurological deterioration causes aberrant phosphatidylcholine flipping in plasma membranes.
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Direct experiments with human ATP8A2 expressed in mouse DKO cells support PS rather than PC transport; the paper separately models ATP8A1, not ATP8A2.
"WT hATP8A2 and hATP11C translocated NBD-PS, but not NBD-PC"