Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Mitochondrial peptide BRAWNIN is essential for vertebrate respiratory complex III assembly.
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BRAWNIN is an inner-membrane microprotein essential for complex III assembly in human cells, zebrafish and mouse fibroblasts.
"In zebrafish, Brawnin deletion causes complete CIII loss, resulting in severe growth retardation, lactic acidosis and early death."
Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.
Defining the interactome of the human mitochondrial ribosome identifies SMIM4 and TMEM223 as respiratory chain assembly factors.
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C12ORF73 is part of an early complex III assembly intermediate with UQCC1, UQCC2 and SMIM4 that binds newly synthesised cytochrome b.
"As expected, both immunoisolations clearly enriched newly synthesized CYTB."
Mitochondrial microproteins link metabolic cues to respiratory chain biogenesis.
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BRAWNIN and SMIM4 join COMA subunits to form the COMB complex, which stabilises and chaperones nascent cytochrome b.
"Furthermore, when nuclear CIII subunits are limiting, COMB is required to chaperone nascent CYTB to prevent OXPHOS collapse."
Knockdown of BRAWNIN minimally affect mitochondrial complex III assembly in human cells.
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Knockout, but not knockdown, of BRAWNIN impairs complex III assembly in human cells.
"although only a minimal amount of BRAWNIN was required for CIII assembly"
The AMPK-related kinase NUAK1 controls cortical axons branching by locally modulating mitochondrial metabolic functions.