Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Combined Automated Annotation using Multiple IEA Methods
Quantitative actin folding reactions using yeast CCT purified via an internal tag in the CCT3/gamma subunit.
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CCT/TRiC is an ATP-dependent folding machine for actin and tubulin.
"The eukaryotic cytosolic chaperonin CCT is an essential ATP-dependent protein folding machine whose action is required for folding the cytoskeletal proteins actin and tubulin"
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Purified yeast CCT catalyzes actin folding.
"Yeast CCT catalyses the folding of yeast ACT1p and human beta-actin with nearly identical rate constants and yields."
Physiological effects of unassembled chaperonin Cct subunits in the yeast Saccharomyces cerevisiae.
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CCT is built from eight distinct subunits Cct1p-Cct8p per ring.
"Eukaryotic chaperonins, the Cct complexes, are assembled into two rings, each of which is composed of a stoichiometric array of eight different subunits, which are denoted Cct1p-Cct8p."
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The CCT subunits are functionally non-equivalent.
"These results provide evidence for functional differences among Cct subunits and for physiological properties of unassembled subunits."
Somatic increase of CCT8 mimics proteostasis of human pluripotent stem cells and extends C. elegans lifespan.
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A single CCT8 subunit is rate-limiting for TRiC/CCT assembly, and increased complex prevents mutant Huntingtin aggregation.
"ectopic expression of a single subunit (CCT8) is sufficient to increase TRiC/CCT assembly"
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Somatic CCT8 increase extends C. elegans lifespan TRiC/CCT-dependently.
"increased expression of CCT8 in somatic tissues extends Caenorhabditis elegans lifespan in a TRiC/CCT-dependent manner"
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CCT8 increase corrects age-associated proteostasis decline in worm HD models.
"ameliorates the age-associated demise of proteostasis and corrects proteostatic deficiencies in worm models"
Systemic regulation of mitochondria by germline proteostasis prevents protein aggregation in the soma of C. elegans.
A genomewide RNAi screen for genes that affect the stability, distribution and function of P granules in Caenorhabditis elegans.
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A genome-wide RNAi screen identified 173 P-granule genes; cct-8 was a hit, and UniProt curates cct-8 as required for correct pgl-1 localization.
"we report on a genomewide RNAi screen in C. elegans, which identified 173 genes that affect the stability, localization, and function of P granules"
Genome sequence of the nematode C. elegans: a platform for investigating biology.
PANTHER family PTHR11353 (CHAPERONIN) metadata
UniProt record Q9N358 (TCPQ_CAEEL, T-complex protein 1 subunit theta)
Falcon deep research synthesis for C. elegans cct-8
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Theta is a low-ATPase subunit that contributes substrate binding, allostery, and assembly rather than bulk ATP consumption.
"CCT-8 occupies a defined position in the low-ATPase hemisphere and plays a specialized role in substrate binding, allosteric regulation, and complex assembly"