Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping
Gene Ontology annotation based on curation of immunofluorescence data
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
The 2-5A/RNase L/RNase L inhibitor (RLI) [correction of (RNI)] pathway regulates mitochondrial mRNAs stability in interferon alpha-treated H9 cells.
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A fraction of RNase L and ABCE1/RLI localizes to mitochondria.
"We found that a fraction of cellular RNase L and RLI is localized in the mitochondria."
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RNase L/RLI activity contributes to interferon-dependent mitochondrial mRNA regulation.
"These results demonstrate that IFNalpha exerts its antiproliferative effect on H9 cells at least in part via the degradation of mitochondrial mRNAs by RNase L."
Basic residues in the nucleocapsid domain of Gag are required for interaction of HIV-1 gag with ABCE1 (HP68), a cellular protein important for HIV-1 capsid assembly.
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HIV-1 Gag interacts with ABCE1/HP68 during immature capsid assembly.
"In primate cells, ABCE1 associates with Gag polypeptides present in immature capsid assembly intermediates."
Defining the membrane proteome of NK cells.
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The membrane fraction proteome includes many non-integral proteins.
"The remaining species were largely involved in cellular processes and molecular functions that could be predicted to be transiently associated with membranes."
The role of ABCE1 in eukaryotic posttermination ribosomal recycling.
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ABCE1 dissociates eukaryotic post-termination ribosomal complexes.
"ABCE1 dissociates post-TCs into free 60S subunits and mRNA- and tRNA-bound 40S subunits."
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ABCE1 is an NTPase whose hydrolysis activity is required for recycling.
"It can hydrolyze ATP, GTP, UTP, and CTP. NTP hydrolysis by ABCE1 is stimulated by post-TCs and is required for its recycling activity."
Dissociation by Pelota, Hbs1 and ABCE1 of mammalian vacant 80S ribosomes and stalled elongation complexes.
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ABCE1 and Pelota are required for mammalian stalled-ribosome dissociation.
"Pelota/Hbs1 also induced dissociation of ECs and release of peptidyl-tRNA, but only in the presence of ABCE1."
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ABCE1 also participates in recycling vacant 80S ribosomes.
"ABCE1/Pelota/Hbs1 also dissociated vacant 80S ribosomes, which stimulated 48S complex formation, suggesting that Pelota/Hbs1 have an additional role outside of NGD."
Host interactions of Chandipura virus matrix protein.
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ABCE1 was identified as a host interactor of Chandipura virus matrix protein.
"The present study aims to screen the human fetal brain cDNA library for interactors of CHPV M protein using yeast two-hybrid system."
Ubiquitination of ABCE1 by NOT4 in Response to Mitochondrial Damage Links Co-translational Quality Control to PINK1-Directed Mitophagy.
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Mitochondrial damage recruits ABCE1 to mitochondrial outer membrane-associated mRNP quality-control complexes.
"Mitochondrial damage causes stalled translation of complex-I 30 kDa subunit (C-I30) mRNA on MOM, triggering the recruitment of co-translational quality control factors Pelo, ABCE1, and NOT4 to the ribosome/mRNA-ribonucleoprotein complex."
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NOT4-mediated ubiquitination of ABCE1 contributes to mitophagy signaling.
"Damage-induced ubiquitination of ABCE1 by NOT4 generates poly-ubiquitin signals that attract autophagy receptors to MOM to initiate mitophagy."
A reference map of the human binary protein interactome.
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High-throughput interactome evidence reports ABCE1 protein interactions.
"The dataset, versioned HI-III-20 (Human Interactome obtained from screening Space III, published in 2020), contains 52,569 verified PPIs involving 8,275 proteins"
OpenCell: Endogenous tagging for the cartography of human cellular organization.
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OpenCell provides high-throughput localization and interaction context for human proteins.
"We combined genome engineering, confocal live-cell imaging, mass spectrometry and data science to systematically map the localization and interactions of human proteins."
Cloning and characterization of a RNase L inhibitor. A new component of the interferon-regulated 2-5A pathway.
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ABCE1/RLI inhibits RNase L activity.
"Its expression in reticulocyte extracts antagonizes the 2-5A binding ability and the nuclease activity of endogenous RNase L or the cloned 2DR polypeptide."
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ABCE1/RLI can suppress an interferon antiviral effect mediated through RNase L.
"The overexpression of RLI in stably transfected HeLa cells inhibits the antiviral activity of IFN on encephalomyocarditis virus but not on vesicular stomatitis virus."
ABCE1 binds RNASEL, inhibiting it
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ABCE1 directly interacts with RNASEL and inhibits its endoribonuclease activity.
"ATP-binding cassette sub-family E member 1 (ABCE1, aka RNase L inhibitor, RLI) directly interacts with RNASEL and inhibits its endoribonuclease activity"
Ribosome-associated quality control
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ABCE1 splits non-stop or stalled cytosolic ribosomes after PELO/HBS1L action.
"HBS1L hydrolyzes GTP and dissociates from PELO and the ribosome, exposing a site on PELO to which ABCE1 binds."
ABCE1:ATP binds PELO:HBS1L-1:GTP:80S ribosome:non-stop mRNA:peptidyl-tRNA with nascent peptide and HBS1L-1:GDP is released
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HBS1L dissociation permits ABCE1 binding to a PELO-containing stalled ribosome.
"The dissociation exposes a surface on PELO for ABCE1 to bind and allows the central domain of PELO to move towards the peptidyl-tRNA in P site of the 80S ribosome"
ABCE1:PELO:80S Ribosome:non-stop mRNA:peptidyl-tRNA with elongating peptide dissociates yielding ABCE1:40S ribosomal subunit, PELO, and 60S ribosomal subunit:peptidyl-tRNA
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ABCE1 hydrolyzes ATP to split a PELO-bound stalled 80S ribosome.
"ABCE1 bound to PELO near the P site of the 80S ribosome hydrolyzes ATP, causing dissociation of the 80S ribosome into 40S and 60S ribosomal subunits"
Deep research on ABCE1 function
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ABCE1 is a conserved Fe-S ABC ATPase whose core function is ATP-driven eukaryotic ribosome recycling.
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ABCE1 functions with eRF1/eRF3 in canonical post-termination recycling and with PELO/HBS1L in stalled-ribosome rescue.
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RNase L inhibition and mitochondrial stress-associated relocalization are supported but are not the central conserved function.
Sequence-level bioinformatics analysis of ABCE1 (transmembrane, NBD, and Fe-S architecture)
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ABCE1 has no transmembrane region (0 UniProt TRANSMEM features; Kyte-Doolittle max 19-residue window 1.54, below the 1.6 TM-helix threshold), consistent with a soluble cytosolic ATPase rather than a membrane transporter.
"The curated UniProt feature table for P61221 lists zero TRANSMEM features, and an independent Kyte-Doolittle scan (window 19) finds no window reaching the 1.6 transmembrane-helix threshold (global maximum 1.54 at residue 64), confirming ABCE1 has no transmembrane region and is a soluble cytosolic protein."
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Two Walker A P-loop motifs map to the UniProt ATP-binding sites (110-117 and 379-386), confirming two nucleotide-binding domains.
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The N-terminal region carries nine cysteines across the two UniProt-annotated ferredoxin-type domains, consistent with coordination of two [4Fe-4S] clusters.
OpenScientist evaluation of ABCE1 membrane annotation