pgr1 (SPBC17A3.07, UniProt P78965) – notes

Glutathione reductase of S. pombe; module roles: GSR step in glutathione_synthesis_gamma_glutamyl_cycle and glutathione_thioredoxin_redox_systems. S. cerevisiae ortholog GLR1 (review: genes/yeast/GLR1).

Evidence

• Activity/gene: PMID:9287302
• Essential, unlike budding yeast: PMID:9287302
• Overexpression protects against menadione but not H2O2 (basis of the NOT GO:0070301): PMID:9287302
• Pap1-dependent stress induction: PMID:9287302
• Localisation: single start codon (no GLR1-like alternative initiation) yet dual location PMID:16950927; PMID:16950927. Organellar fraction was crude; "most likely in mitochondria". ORFeome HDA also says mitochondrion PMID:16823372.
• Essential role = Fe-S enzyme maintenance; suppressed by mitochondrial Trx2: PMID:16950927
• FAD cofactor/homodimer by similarity to GLR1 [UniProt:P78965].

Comparison

• Core function matches yeast GLR1 review (GO:0004362; BP cell redox homeostasis + glutathione metabolic process; cytosol + mitochondrion). S. pombe-specific differences: essential gene; single translation product (no alternative start).
• PomBase GO-CAM 68b0f0d000008341 places pgr1 only in mitochondrion (IDA PMID:16950927) and part_of GO:0006749; the module annoton puts it in cytosol. Both compartments are supported.