Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping
Combined Automated Annotation using Multiple IEA Methods
Deep research report on B. subtilis amyE alpha-amylase
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Crystal structure at 2.50 angstroms resolution shows calcium binding sites and active site architecture
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Identification of two calcium binding sites per subunit
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Characterization of active site residues (Asp217 nucleophile, Glu249 proton donor, Asp310 transition state stabilizer)
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Complex with maltopentaose showing substrate binding
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Sec-mediated secretion pathway with discrete secretion zones in cell wall
UniProt entry for B. subtilis alpha-amylase AmyE
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Signal peptide residues 1-27
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Propeptide residues 28-41
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Mature chain residues 42-659
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Two calcium binding sites with coordinating residues identified
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GH13 family membership
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CBM26 carbohydrate-binding module