Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on curation of immunofluorescence data
Combined Automated Annotation using Multiple IEA Methods
Regulation of human separase by securin binding and autocleavage.
Human full-length Securin is a natively unfolded protein.
Systematic analysis of human protein complexes identifies chromosome segregation proteins.
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The study used tagged proteins and affinity-purification mass spectrometry to map human protein complexes.
"protein localization, and tandem-affinity purification-mass spectrometry"
PP2A delays APC/C-dependent degradation of separase-associated but not free securin.
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Separase-associated securin is stabilized by PP2A-dependent dephosphorylation.
"PP2A keeps separase-associated securin in a dephosphorylated state, thereby selectively stabilizing this protease-bound pool of the anaphase inhibitor."
A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
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Quantitative AP-MS mapped interaction stoichiometries and cellular abundance in human cells.
"Using quantitative proteomics, we detect specific interactions, estimate interaction stoichiometries, and measure cellular"
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
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BioPlex generated cell-line-specific human interaction networks using affinity-purification mass spectrometry.
"Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks."
Structural basis of human separase regulation by securin and CDK1-cyclin B1.
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Cryo-EM directly resolves the human separase-securin complex.
"We determined the structures of a wild-type separase-securin complex"
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PTTG1 motifs inhibit separase at the catalytic and substrate-docking sites.
"Studies of truncated securin mutants revealed that this hydrophobic stretch is required for high-affinity securin-separase binding and inhibition of separase activity"
hpttg, a human homologue of rat pttg, is overexpressed in hematopoietic neoplasms. Evidence for a transcriptional activation function of hPTTG.
Pituitary tumor-transforming gene protein associates with ribosomal protein S10 and a novel human homologue of DnaJ in testicular cells.
Association of cell cycle proteins with the APC/C:Cdh1 complex
Degradation of multiubiquitinated cell cycle proteins
Association of Securin with Cdc20:APC/C complex
Ubiquitination of Securin by phospho-APC/C:Cdc20 complex
Ubiquitination of cell cycle proteins targeted by the APC/C:Cdh1complex
Degradation of multiubiquitinated Securin
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Ubiquitinated securin is degraded by the proteasome.
"Following ubiquitination, securin is degraded by the 26S proteasome."
PTTG1 (Securin) sequesters ESPL1 (Separase)
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PTTG1 sequesters ESPL1 and prevents premature cohesin cleavage.
"PTTG1 sequesters ESPL1 and block its catalytic site, preventing it from cleaving centromeric cohesin and causing premature separation of sister chromatids"
Identification of a vertebrate sister-chromatid separation inhibitor involved in transformation and tumorigenesis.
Securin is required for chromosomal stability in human cells.
Critical differences between isoforms of securin reveal mechanisms of separase regulation.
UniProtKB record for human PTTG1 (O95997)
Manual deep-research synthesis for human PTTG1
Manual PTTG1 curation notes