Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
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UniProt keywords include ATP-binding, Competence, Membrane, Nucleotide-binding, Transport
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping
Microarray analysis of the Bacillus subtilis K-state genome-wide expression changes dependent on ComK
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comGA is part of the ComK regulon expressed during competence development
"In Bacillus subtilis, the competence transcription factor ComK activates its own transcription as well as the transcription of genes that encode DNA transport proteins"
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ComK activates transcription of genes encoding DNA transport proteins including comGA
"the competence transcription factor ComK activates its own transcription as well as the transcription of genes that encode DNA transport proteins"
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Approximately 10% of cells in a culture express ComK and become competent
"ComK is expressed in about 10% of the cells in a culture grown to competence"
Whole-genome analysis of genes regulated by the Bacillus subtilis competence transcription factor ComK
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ComK is required for comGA expression and establishment of competence for genetic transformation
"The Bacillus subtilis competence transcription factor ComK is required for establishment of competence for genetic transformation"
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comGA is among the genes with ComK-dependent expression
"we found many genes or operons whose ComK dependence was not known previously"
Deep research review of comGA function and localization
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ComGA is an AAA+ ATPase homologous to type II secretion system GspE ATPases
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Walker A/B motifs are essential for function with mutations abolishing DNA binding
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ComGA localizes to cell poles through diffusion-capture mediated by ComEB
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ComGA dynamics increase upon DNA addition consistent with pilus activity
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Deletion of comGA abolishes competence pilus formation and transformation
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Competence pili can form at multiple sites but cytosolic DNA entry is polar via ComEC