PA5017 (DipA, Q9HUF2) review notes

Review session 2026-11-05 (PseudoCAP IBA-seed protocol, tier 2)

Fetched with just fetch-gene PSEAE Q9HUF2 --alias PA5017; accession matches the worklist.
No deep-research provider used. PMID:22493016 has full text cached (PMC3370607) and was read.

Literature

PMID:22493016
PDE assay: His-tagged DipA purified from P. aeruginosa, bis-pNPP and c-di-GMP substrates
PMID:22493016.
Low activity is attributed to partial insolubility; EAL1 and EAL2 alanine mutants fail to
complement swarming or dispersion and fail to suppress PA4843-induced aggregation.
The GGDEF domain is degenerate (ASNEF) and no cyclase activity was detected.
Dispersion phenotype is cue-general PMID:22493016

Annotation decisions

ACCEPT: GO:0071111 (IDA, IBA, IEA).
KEEP_AS_NON_CORE: GO:0043200 response to amino acid (IMP); a cue-general dispersion phenotype.
Action counts: ACCEPT 3, KEEP_AS_NON_CORE 1. No source defects.

PseudoCAP IBA-seed assessment

Shares PTN002403463 with mucR (PA1727, Q9I310); verdicts kept consistent, see mucR notes. No
node-level defect. The node sits in PTHR44757:SF2 and is distinct from the PTN002105365 PDE node
covered in the rocR review and the PTN002895351 node covered in wspR.

PTN node	node term	n_seeds	leaf IBAs	seed annotation (term, evidence, PMID)	verdict	rationale	other seeds	IBD stands without this seed?
PTN002403463	GO:0071111 cyclic-guanylate-specific phosphodiesterase activity	3	14 (5 taxa)	GO:0071111, IDA, PMID:22493016 (PseudoCAP)	SOUND	Full text: purified DipA hydrolyses c-di-GMP and bis-pNPP; EAL motifs required.	E. coli PdeR P77334 (IDA PMID:17010156, EXP PMID:23708798), mucR Q9I310 (IDA PMID:23729646)	Yes

Clade note (no defect recorded): leaf PA4929 (Q9HUN5) in this clade has MHYT and GGDEF domains
but no EAL domain in UniProt/InterPro, so the PDE IBA on that leaf is doubtful. This is a
leaf-level exception (a NOT for that leaf) rather than an unsound node, and PA4929 is not in
this batch.