Combined Automated Annotation using Multiple IEA Methods
file:PSEPK/moeA/moeA-uniprot.txt
UniProtKB entry for moeA
UniProt assigns Q88L14 molybdopterin molybdenumtransferase activity, EC 2.10.1.1, producing Mo-molybdopterin from adenylyl-MPT and molybdate.
"CC -!- FUNCTION: Catalyzes the insertion of molybdate into adenylated
CC molybdopterin with the concomitant release of AMP.
CC {ECO:0000256|ARBA:ARBA00002901, ECO:0000256|RuleBase:RU365090}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=adenylyl-molybdopterin + molybdate = Mo-molybdopterin + AMP +
CC H(+); Xref=Rhea:RHEA:35047, ChEBI:CHEBI:15378, ChEBI:CHEBI:36264,
CC ChEBI:CHEBI:62727, ChEBI:CHEBI:71302, ChEBI:CHEBI:456215;
CC EC=2.10.1.1; Evidence={ECO:0000256|ARBA:ARBA00047317};"
file:PSEPK/moeA/moeA-goa.tsv
QuickGO GOA annotations for moeA
The fetched table contains the exact MoeA activity annotation reviewed here.
The report explicitly identifies the lack of target-specific experimental evidence.
"- No *P. putida* KT2440-specific biochemical or structural study of PP_2123 was found; functional assignment relies on high-confidence orthology to *E. coli* MoeA and conserved domain architecture. Direct enzymatic characterization or a KT2440 knockout phenotype would confirm substrate specificity (Mo vs W) and downstream molybdoenzyme dependencies in this organism."