E2-RING expansion of the NEDD8 cascade confers specificity to cullin modification.
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Biochemical and cellular experiments identify full-length UBE2F as a NEDD8-conjugating E2, not a ubiquitin E2.
"the previously uncharacterized E2 UBE2F is a NEDD8-conjugating enzyme in vitro
and in vivo."
Inhibition of a NEDD8 Cascade Restores Restriction of HIV by APOBEC3G.
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Full-length UBE2F indirectly enables a ubiquitin-ligase pathway through cullin neddylation; this is distinct from intrinsic ubiquitin transfer.
"conjugation of NEDD8 to Cullin-5 by the NEDD8-conjugating
enzyme UBE2F is required for HIV Vif-mediated degradation"
Exact UBE2F product-to-reference sequence mapping
Frozen exact-input ProtNLM output and UniProt sequence for F8WDQ9