GO_REF:0000024

Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity

GO_REF:0000033

Annotation inferences using phylogenetic trees

GO_REF:0000052

Gene Ontology annotation based on curation of immunofluorescence data

GO_REF:0000104

Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features

GO_REF:0000107

Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara

GO_REF:0000117

Electronic Gene Ontology annotations created by ARBA machine learning models

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

PMID:15960975

Physical association and coordinate function of the H3 K4 methyltransferase MLL1 and the H4 K16 acetyltransferase MOF.

CHD8 is recovered with WDR5/MLL-associated material, supporting context-dependent association rather than invariant MLL1 stoichiometry.

PMID:17938208

CHD8 associates with human Staf and contributes to efficient U6 RNA polymerase III transcription.

CHD8 contributes to human U6 and IRF3 transcription and is recovered preferentially with H3K4 methylated peptides.

PMID:18378692

CHD8 is an ATP-dependent chromatin remodeling factor that regulates beta-catenin target genes.

Purified human CHD8 remodels nucleosomes and binds WDR5 and beta-catenin; depletion alters beta-catenin-responsive gene expression.

PMID:20085832

Regulation of HOXA2 gene expression by the ATP-dependent chromatin remodeling enzyme CHD8.

CHD8 associates with WDR5, RBBP5 and ASH2L and influences their occupancy and HOXA2 regulation.

PMID:20453063

CHD8 interacts with CHD7, a protein which is mutated in CHARGE syndrome.

CHD8 C-terminal fragments associate with CHD7 in Y2H, tagged co-IP and nuclear BiFC; some CHD7 variants affect the assay types differently.

PMID:22083958

Histone H1 recruitment by CHD8 is essential for suppression of the Wnt-β-catenin signaling pathway.

CHD8 association with beta-catenin and H1 recruitment contribute to repression; detailed bridging mutants were tested in mouse short CHD8.

PMID:23285124

Identification and characterization of FAM124B as a novel component of a CHD7 and CHD8 containing complex.

Both FAM124B alternatives associate with the tested CHD8 C-terminal region; the study does not establish a distinct FAM124B molecular activity.

PMID:24998929

Disruptive CHD8 mutations define a subtype of autism early in development.

Disruptive CHD8 variation and zebrafish perturbation associate with brain enlargement, altered enteric innervation and digestive transit.

PMID:25294932

CHD8 regulates neurodevelopmental pathways associated with autism spectrum disorder in neural progenitors.

Partial CHD8 suppression changes transcription in both directions and affects neurodevelopmental networks.

PMID:26816381

Transcription factors LRF and BCL11A independently repress expression of fetal hemoglobin.

CHD8 is a positive LRF/ZBTB7A-associated protein; this is distinct from assigning all endogenous NuRD results in the paper to CHD8.

PMID:26949739

Identification of Human Neuronal Protein Complexes Reveals Biochemical Activities and Convergent Mechanisms of Action in Autism Spectrum Disorders.

Endogenous neuronal-cell CHD8 complexes include the verified ACOT7 association.

PMID:28533432

The ATP-dependent chromatin remodeling enzymes CHD6, CHD7, and CHD8 exhibit distinct nucleosome binding and remodeling activities.

Human CHD8 hydrolyzes ATP, binds DNA and slides nucleosomes, with linker-DNA requirements distinct from CHD7 and CHD6.

PMID:36537238

CHD8 suppression impacts on histone H3 lysine 36 trimethylation and alters RNA alternative splicing.

CHD8 suppression alters alternative splicing and H3K36me3, and CHD8 associates with HNRNPL in a partly RNA-sensitive complex.

Reactome:R-HSA-5368580

CHD8 binds beta-catenin to negatively regulate WNT-dependent gene expression

The represented CHD8-containing event occurs in the nucleoplasm.

Reactome:R-HSA-9944087

CHD6-9-dependent ATP hydrolysis

The represented CHD8-containing event occurs in the nucleoplasm.

Reactome:R-HSA-9944419

CHD8 and CHD7 bind CTCF

The represented CHD8-containing event occurs in the nucleoplasm.

Reactome:R-HSA-9944450

CHD7 and CHD8 bind FAM124B

The represented CHD8-containing event occurs in the nucleoplasm.

Reactome:R-HSA-9944456

CHD8 binds WDR5

The represented CHD8-containing event occurs in the nucleoplasm.

PMID:19151705

CHD8 suppresses p53-mediated apoptosis through histone H1 recruitment during early embryogenesis.

CHD8/p53/H1 associations and p53-dependent depletion phenotypes support context-dependent transcriptional protection from apoptosis.

PMID:42801681

CHD8-Dependent Chromatin Licensing Sustains Trophoblast Stem Cell Transcriptional Programs.

CHD8 associates with KMT2A in trophoblast contexts and supports chromatin accessibility and transcription-factor occupancy.

PMID:31544921

The internal interaction in RBBP5 regulates assembly and activity of MLL1 methyltransferase complex.

Human RBBP5 full-length 2–538 and its CTD3 region bind dsDNA in purified-protein fluorescence-polarization assays; lysine substitutions reduce binding and nucleosome association. This resolves the partner DNA-contact boundary without assigning the partner activity to CHD8.

PMID:21660059

Crystal structure of the N-terminal region of human Ash2L shows a winged-helix motif involved in DNA binding.

Human full-length ASH2L and its N-terminal winged-helix region bind duplex DNA by EMSA; mutations affect DNA interaction and chromatin occupancy. The reported selection did not identify a strong consensus sequence.