GO_REF:0000002

Gene Ontology annotation through association of InterPro records with GO terms

GO_REF:0000015

Use of the ND evidence code for Gene Ontology (GO) terms

GO_REF:0000033

Annotation inferences using phylogenetic trees

GO_REF:0000116

Automatic Gene Ontology annotation based on Rhea mapping

GO_REF:0000117

Electronic Gene Ontology annotations created by ARBA machine learning models

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

PMID:20489023

A global protein kinase and phosphatase interaction network in yeast.

Large-scale mass-spectrometric mapping of the yeast kinase/phosphatase interaction (KPI) network; the basis for the SGD ISS annotation transferring a Ser/Thr kinase molecular function to KDX1 from its paralog SLT2. Abstract-only in cache; no KDX1-specific catalytic demonstration is available from the abstract.

PMID:9111331

Characterization of a serum response factor-like protein in Saccharomyces cerevisiae, Rlm1, which has transcriptional activity regulated by the Mpk1 (Slt2) mitogen-activated protein kinase pathway.

Original identification of Mlp1 (KDX1) as an Rlm1-associated protein by yeast two-hybrid; overexpression of MLP1 suppresses the caffeine sensitivity of bck1-delta, and mlp1-delta is additive with mpk1-delta, indicating Rlm1 activity is regulated by both Mpk1 and the "Mlp1 MAP kinase-like kinase".

PMID:18055054

A yeast strain biosensor to detect cell wall-perturbing agents.

MLP1/YKL161c is almost undetectable under normal growth but its protein accumulates in large amounts when cell wall integrity is compromised, making the MLP1 promoter an ideal cell-wall-stress reporter.

PMID:18268013

Yeast Mpk1 mitogen-activated protein kinase activates transcription through Swi4/Swi6 by a noncatalytic mechanism that requires upstream signal.

Mpk1 and its pseudokinase paralog Mlp1 (KDX1) use a noncatalytic mechanism to activate FKS2 transcription; activated (phosphorylated) Mpk1 and Mlp1 were detected in a complex with Swi4 and Swi6 at the FKS2 promoter, conferring DNA-binding ability to Swi4 which then recruits Swi6.

PMID:20641022

Transcriptional reporters for genes activated by cell wall stress through a non-catalytic mechanism involving Mpk1 and SBF.

Explicitly describes Mlp1 (KDX1) as "a catalytically inactive paralog of Mpk1"; cell wall stress induction of FKS2/CHA1/YKR013w/YLR042c is only partially dependent on Mlp1 but fully blocked in an mpk1-delta mlp1-delta double mutant, and MLP1 is itself transcriptionally induced by Mpk1 through Rlm1.

PMID:35420390

Hsp90 and phosphorylation of the Slt2(Mpk1) MAP kinase activation loop are essential for catalytic, but not non-catalytic, Slt2-mediated transcription in yeast.

Swi4/Swi6 (SBF) activation in response to cell wall stress is noncatalytic and triggered by binding of phosphorylated forms of both Slt2 and the catalytically inactive pseudokinase Mlp1 (KDX1).

file:yeast/KDX1/KDX1-bioinformatics/RESULTS.md

Reproducible KDX1 and SLT2 sequence-coordinate comparison

The canonical beta3 lysine and DFG aspartate are substituted in Kdx1; the adjacent retained lysine is a different aligned position. Sequence comparison does not itself settle ATP binding or all residual activity.

"The canonical SLT2 ATP-site Lys54 aligns to Arg54 in KDX1."

file:yeast/KDX1/KDX1-hypotheses/cell-cycle-complex-and-nucleotide-binding-functions/openscientist.md

OpenScientist focused report on KDX1 cell-cycle, CDK-complex and ATP-binding assignments

The focused report found no direct KDX1 role in the cell-cycle machinery; apparent cell-cycle support comes indirectly from the SBF partner.

"any cell-cycle connotation arises from SBF's separate G1/S role, not from KDX1 acting on the cell-cycle machinery"
The focused report found no support for placing KDX1 in a cyclin-CDK holoenzyme.

"No evidence KDX1 is in a CDK–cyclin complex"
The focused report treated ATP binding as unresolved because GO:0005524 is rule-based and lacks a direct KDX1 nucleotide-binding assay.

"Rule-based only; no assay. Cannot confirm ATP binding."

file:yeast/KDX1/KDX1-uniprot.txt

UniProt entry P36005 (KDX1)

file:yeast/KDX1/KDX1-deep-research-sgd.md

SGD gene description for KDX1 (yeast)