ALD4 (YOR374W, UniProt P46367) notes

Identity / naming

• Major mitochondrial K+-activated ALDH. Called "ALD7" in Tessier et al. PMID:9675847 and "ALDH2" (commercial enzyme, chromosome XV) in Wang et al. PMID:9473035
• Matrix location [UniProt:P46367]; part of a native mitochondrial dehydrogenase supercomplex PMID:11502169

Activity

• Dual cofactor PMID:9473035; Km acetaldehyde 10 uM with NADP [UniProt:P46367]
• Deletion abolishes K+-ACDH, impairs ethanol growth PMID:9675847

Processes

• Acetate: backup for Ald6p PMID:10919763; PMID:15256563
• Mitochondrial NADPH PMID:19158096
• Lipid: overexpression phenotype only, indirect PMID:39462103

Curation observations

• RCA cytosol is_active_in (from GLUCFERMEN-PWY compartment) contradicts matrix localization: removed.
• Bifid shunt RCA removed. Proposed NEW ethanol catabolic process (IMP, PMID:9675847).
• Mitochondrial nucleoid IDA (PMID:10869431): abstract does not name Ald4p; kept non-core.