Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping
Automatic assignment of GO terms using logical inference
Cloning and characterization of a specific coactivator, ARA70, for the androgen receptor in human prostate cells.
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Original characterization of NCOA4 as ARA70, androgen receptor coactivator
"Using a yeast two-hybrid system, we were able to isolate a ligand-dependent AR-associated protein (ARA70), which functions as an activator to enhance AR transcriptional activity 10-fold in the presence of 10(-10) M dihydrotestosterone or 10(-9) M testosterone, but not 10(-6) M hydroxyflutamide in human prostate cancer DU145 cells"
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Demonstrated ligand-dependent enhancement of AR transcriptional activity
"Using a yeast two-hybrid system, we were able to isolate a ligand-dependent AR-associated protein (ARA70), which functions as an activator to enhance AR transcriptional activity 10-fold in the presence of 10(-10) M dihydrotestosterone or 10(-9) M testosterone, but not 10(-6) M hydroxyflutamide in human prostate cancer DU145 cells"
Isolation and characterization of ARA160 as the first androgen receptor N-terminal-associated coactivator in human prostate cells.
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Paper primarily about ARA160/TMF, not NCOA4
"we identified an androgen-enhanced AR N-terminal-associated protein ARA160, which consists of 1,093 amino acids with an apparent molecular mass of 160 kDa"
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Mentions cooperative function between ARA160 and ARA70
"Our data further suggest that this AR N-terminal coactivator can function cooperatively with AR C-terminal coactivator, ARA70, in PC-3 cells"
Selective VPS34 inhibitor blocks autophagy and uncovers a role for NCOA4 in ferritin degradation and iron homeostasis in vivo.
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Seminal paper identifying NCOA4 as ferritinophagy cargo receptor
"NCOA4 directly binds ferritin heavy chain-1 (FTH1) to target the iron-binding ferritin complex with a relative molecular mass of 450,000 to autolysosomes following starvation or iron depletion"
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NCOA4 directly binds FTH1 to target ferritin to autolysosomes
"NCOA4 directly binds ferritin heavy chain-1 (FTH1) to target the iron-binding ferritin complex with a relative molecular mass of 450,000 to autolysosomes"
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Ncoa4-/- mice show iron accumulation in splenic macrophages
"Ncoa4(-/-) mice exhibit a profound accumulation of iron in splenic macrophages, which are critical for the reutilization of iron from engulfed red blood cells"
Architecture of the human interactome defines protein communities and disease networks.
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Multimodal cell maps as a foundation for structural and functional genomics.
Coactivators are recruited to liganded PPARG:RXRA heterodimer
Deep research synthesis on NCOA4 (2023-2024 reviews)
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NCOA4 is the principal ferritin cargo receptor for ferritinophagy
"Serves as the selective cargo receptor that binds ferritin (FTH1) and delivers it to autophagic/lysosomal degradation"
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N-terminal region mediates nuclear receptor coactivation (secondary function)
"N-terminal coiled-coil / nuclear-receptor-binding region (~1-237); C-terminal ferritin-binding domain (~238-614)"
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Ferritinophagy positively regulates ferroptosis by increasing labile iron
"Excessive ferritinophagy increases labile Fe2+ and lipid peroxidation, sensitizing cells to ferroptosis"