Annotation inferences using phylogenetic trees

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

file:human/ALG5/ALG5-uniprot.txt

UniProtKB entry Q9Y673 (ALG5_HUMAN)

PMID:10359825

A mutation in the human ortholog of the Saccharomyces cerevisiae ALG6 gene causes carbohydrate-deficient glycoprotein syndrome type-Ic.

The human ALG5 ortholog was cloned and functionally characterized; its cDNA partially complements the S. cerevisiae alg5 deficiency, and ALG5 encodes the dolichyl phosphate glucosyltransferase acting in LLO biosynthesis. ALG5 itself was not mutated in the CDGS-Ic patients (the causal gene was ALG6).

PMID:19946888

Defining the membrane proteome of NK cells.

Large-scale MS characterization of the NK (YTS) cell membrane proteome identified ALG5 among 1843 proteins; supports bulk membrane localization only.

PMID:32353859

A SARS-CoV-2 protein interaction map reveals targets for drug repurposing.

Proteome-wide SARS-CoV-2-human interactome identified an ALG5-ORF3a (P0DTC3) physical interaction; source of an IntAct protein binding IPI.

PMID:32838362

Virus-Host Interactome and Proteomic Survey Reveal Potential Virulence Factors Influencing SARS-CoV-2 Pathogenesis.

Virus-host interactome/proteomic survey reporting ALG5 interaction with SARS-CoV-2 ORF3a; source of an IntAct protein binding IPI.

PMID:33060197

Comparative host-coronavirus protein interaction networks reveal pan-viral disease mechanisms.

Comparative host-coronavirus interactome reporting the ALG5-ORF3a interaction; source of an IntAct protein binding IPI.

PMID:36217030

A comprehensive SARS-CoV-2-human protein-protein interactome reveals COVID-19 pathobiology and potential host therapeutic targets.

Comprehensive SARS-CoV-2-human interactome reporting the ALG5-ORF3a interaction; source of an IntAct protein binding IPI.

Reactome:R-HSA-446214

Synthesis of dolichyl-phosphate-glucose

Reactome reaction in which ALG5 (dolichyl-phosphate beta-glucosyltransferase) catalyzes UDP-glucose + dolichyl phosphate to form Dol-P-Glc on the cytosolic face of the ER membrane.

Reactome:R-HSA-480985

Synthesis of dolichyl-phosphate-glucose

Reactome pathway describing synthesis of Dol-P-Glc and its role as glucose donor in three steps of N-glycan precursor biosynthesis.