Gene Ontology annotation through association of InterPro records with GO terms

Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity

GO_REF:0000033

Annotation inferences using phylogenetic trees

GO_REF:0000044

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

GO_REF:0000107

Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

PMID:16237761

Screening of hepatocyte proteins binding to F protein of hepatitis C virus by yeast two-hybrid system.

PMID:20378551

Golgi N-glycosyltransferases form both homo- and heterodimeric enzyme complexes in live cells.

ST6GAL1 (SiaT) forms disulfide-mediated and non-covalent homodimers in live Golgi membranes (>90% of molecules homodimeric) and a trans-Golgi heterodimer with B4GALT1; localizes to Golgi membranes.

PMID:21081508

Universal phosphatase-coupled glycosyltransferase assay.

Kinetic parameters of human sialyltransferase ST6GAL1 were measured using a phosphatase-coupled assay.

PMID:23999306

The structure of human α-2,6-sialyltransferase reveals the binding mode of complex glycans.

Crystal structures of human ST6Gal-I define the GT-A variant fold, CMP product/inhibitor binding, complex-glycan binding mode, and an SN2 inversion, substrate-assisted catalytic mechanism.

PMID:25733881

Controlled tetra-Fc sialylation of IVIg results in a drug candidate with consistent enhanced anti-inflammatory activity.

ST6GAL1 alpha-2,6-sialylation of IgG Fc N-glycans yields tetra-Fc-sialylated IVIg with enhanced anti-inflammatory activity.

PMID:27872474

Multi-level glyco-engineering techniques to generate IgG with defined Fc-glycans.

ST6GAL1 is used in the glycosylation pathway to install alpha-2,6 sialic acid on IgG Fc glycans (UniProt PATHWAY evidence for protein glycosylation).

PMID:29133956

Network inference from glycoproteomics data reveals new reactions in the IgG glycosylation pathway.

Glycoproteomics network inference places ST6GAL1 in the IgG glycosylation pathway and supports its trans-Golgi role.

PMID:35789385

The incorrect use of CD75 as a synonym for ST6GAL1 has fostered the expansion of commercial 'ST6GAL1' antibodies that do not recognize ST6GAL1.

CD75 is a carbohydrate epitope generated by ST6GAL1, not the ST6GAL1 protein; conflation of the two has produced commercial antibodies that do not recognize ST6GAL1.

PMID:36280670

A universal glycoenzyme biosynthesis pipeline that enables efficient cell-free remodeling of glycans.

Recombinant ST6GAL1 installs alpha-2,6 sialic acid in a cell-free glycan remodeling pipeline (EC 2.4.3.1 catalytic activity).

PMID:37632720

Divergent Enzymatic Assembly of a Comprehensive 64-Membered IgG N-Glycan Library for Functional Glycomics.

ST6GAL1 is used for alpha-2,6 sialylation in the divergent enzymatic assembly of a 64-membered IgG N-glycan library.

Reactome:R-HSA-4085033

ST6GAL1,2 transfer Neu5Ac to terminal Gal (alpha-2,6 link)

Reactome:R-HSA-9683673

Maturation of protein 3a

Reactome:R-HSA-9683769

O-glycosylation of 3a is terminated

Reactome:R-HSA-9694548

Maturation of spike protein

Reactome:R-HSA-9697018

Addition of sialic acids on some Spike glycosyl sidechains

Reactome:R-HSA-975902

ST6GAL1 transfers Neu5Ac to terminal Gal of N-glycans

Reactome:R-HSA-977068

Termination of O-glycan biosynthesis

Reactome:R-HSA-977071

ST6GAL1 transfers sialic acid to Tn antigens to form sTn antigens