Gene Ontology annotation through association of InterPro records with GO terms.
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt.
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods.
A catalytic role of XoxF1 as La3+-dependent methanol dehydrogenase in Methylobacterium extorquens strain AM1
Contrasting in vitro and in vivo methanol oxidation activities of lanthanide-dependent alcohol dehydrogenases XoxF1 and ExaF from Methylobacterium extorquens AM1.
Lanthanide-dependent alcohol dehydrogenases require an essential aspartate residue for metal coordination and enzymatic function
Falcon deep research synthesis on xoxF1 (C5B120) lanthanide-dependent methanol dehydrogenase function, cofactors, localization, and regulation in M. extorquens AM1
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"XoxF-type MDHs (including XoxF1) are active when Ln(III) (e.g., La(III), Ce(III), Pr(III), Nd(III)) are available and oxidize methanol → formaldehyde"
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"XoxF1 is a PQQ-dependent dehydrogenase whose active site requires Ln(III) rather than Ca(II), distinguishing it from Ca-dependent MxaFI"
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"lanthanides in the active site act as Lewis acids that facilitate hydride transfer to PQQ during alcohol oxidation"
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"XoxF purified from lanthanide-grown cells incorporated ~1.24 atoms of La and lacked Ca"
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"AM1 xoxF1 is repeatedly described/annotated as periplasmic, consistent with the general statement that methylotrophic PQQ-ADHs are periplasmic enzymes"
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"A schematic model explicitly places XoxF1 in the periplasm catalyzing methanol oxidation in the presence of lanthanum"
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"XoxG (a cytochrome cL-like protein) is described as transferring electrons from PQQ to downstream components of the respiratory chain, and XoxJ is a periplasmic binding protein implicated in XoxF activation"
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"with Ln present, xox genes are upregulated and mxa genes are repressed; with Ln absent, mxaFI dominates"
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"loss of either phenocopies loss of xoxF1/xoxF2 for Ln-dependent methanol growth"
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"Maximal growth rate and yield at ≥1 μM La"
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"411 distinct MDH sequences were recovered; all were XoxF-type, and no Ca-dependent MxaF MDHs were found"
Perplexity deep research synthesis on xoxF1 function and regulation
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"the immediate physiological electron acceptor is a specific periplasmic cytochrome c designated XoxG (cytochrome cL)"
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"The *xoxG* gene is invariably located immediately adjacent to *xoxF1* in the bacterial genome as part of the xoxFGJ operon"
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"upregulates expression of *xoxF* genes while simultaneously repressing expression of *mxa* genes encoding the calcium-dependent methanol dehydrogenase"
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"The *xoxF* gene is distributed across five phylogenetically distinct clades (XoxF1 through XoxF5) with differential environmental distribution and substrate specificity"
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"an affinity constant (Km) for methanol of 1.4 ± 0.6 μM"