"significantly impaired the growth of the ycdH mutant under conditions of zinc limitation"

"Mutation of yciA or yciC, singly or in combination, had little effect on growth of the wild-type strain"

"Zur binds to both of these sites to mediate strong, zinc-dependent repression of yciC"

"yciC, encodes an abundant protein postulated to function as a metallochaperone"

"Bacillus subtilis YciC zinc metallochaperone (here renamed ZagA for ZTP activated GTPase A)"

"Here, we demonstrate that the Bacillus subtilis YciC zinc metallochaperone (here renamed ZagA for ZTP activated GTPase A) supports de novo folate biosynthesis under conditions of zinc limitation, and interacts directly with the zinc-dependent GTP cyclohydrolase IA, FolE (GCYH-IA)"

"ZTP, a signal of 10-formyl-tetrahydrofolate (10f-THF) deficiency in bacteria, transiently accumulates as FolE begins to fail, stimulates the interaction between ZagA and FolE, and thereby helps to sustain folate synthesis despite declining zinc availability."

"At the onset of zinc limitation, the purine biosynthetic intermediate 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR), also known as ZMP, accumulates and is known to be phosphorylated to ZTP. The Zur-regulated metallochaperone ZagA is activated by ZTP to bind FolE, and this interaction likely allows delivery of zinc to FolE to sustain folate synthesis."

"this interaction likely allows delivery of zinc to FolE to sustain folate synthesis"

"At the onset of zinc limitation, the purine biosynthetic intermediate 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR), also known as ZMP, accumulates and is known to be phosphorylated to ZTP"

"The Cluster of Orthologous Groups (COG) COG0523 subfamily, defined by the conserved CxCC (C, Cys; x, any amino acid) primary structure motif responsible for high affinity metal binding"

"the G3E family is thought to utilize GTP hydrolysis to thermodynamically drive and/or regulate metallocofactor assembly"

"the extent to which ZagA might catalyze subunit exchange in a FolE oligomer in a ZTP/GTP-hydrolysis dependent manner"

"The Cluster of Orthologous Groups (COG) COG0523 subfamily, defined by the conserved CxCC (C, Cys; x, any amino acid) primary structure motif responsible for high affinity metal binding, 28 , 29 comprises the last known subfamily of the G3E P-loop superfamily, and is far less understood"

"zinc chaperone that directly transfers zinc cofactor to target proteins...best-characterized interactions is with FolE, a zinc-dependent GTP cyclohydrolase I critical for de novo folate biosynthesis"

"belongs to the COG0523 subfamily of putative P-loop GTPases"