SLY1 / SLEEPY1 (At4g24210, UniProt Q9STX3) curation notes

Identity

• just fetch-gene ARATH SLY1 first resolved to the wrong protein: Q9SL48 (SEC1-family transport protein SLY1, At2g17980). Deleted and refetched by accession: just fetch-gene ARATH Q9STX3 --alias SLY1.
• Q9STX3 GID2_ARATH "F-box protein GID2" (AltName Protein SLEEPY 1); Name=GID2, Synonyms=SLY1; At4g24210. gene_symbol set to SLY1 (TAIR symbol).
• PANTHER PTHR47750 (F-BOX PROTEIN SNE) per UniProt; PAINT IBDs in GOA come from older PTHR12874 nodes PTN000305657 (eukaryotic F-box) and PTN001767709 (land-plant GID2/SLY1; rice GID2 Q7XAK4).

Key findings (with provenance)

• F-box protein, positive regulator of GA signaling [PMID:12724538 "Positional cloning of SLY1 revealed that it encodes a putative F-box protein"; "sly1 mutations cause a significant increase in RGA protein accumulation even after GA treatment"; "an rga null allele partially suppresses the sly1-10 mutant phenotype"].
• Direct DELLA binding via GRAS domain PMID:15155881; nuclear PMID:15155881.
• SCF composition and substrate specificity [PMID:15161962 "SLY1 is a subunit of an Arabidopsis SCF SLY1 E3 ubiquitin ligase complex that contains the highly conserved ASK1/2 and AtCUL1 subunits"; "SLY1 (the wild-type form) and sly1gar2-1 both confer substrate specificity on this complex via specific binding to the DELLA proteins"].
• GA-GID1 promotes RGA-SLY1 interaction PMID:17194763.
• SNE/SLY2 paralog partially redundant [PMID:15308775; PMID:21163960 "The FLAG-SLY1 fusion protein was found to coimmunoprecipitate with the GA receptor HA-GA-INSENSITIVE DWARF1b (GID1b)"].
• Cryo-EM: PMID:40542507; PMID:40768360.
• Seed dormancy/germination: PMID:17384169.
• Falcon report (SLY1-deep-research-falcon.md) concurs: "SLY1 is a substrate-recognition adaptor, not a gibberellin-metabolizing enzyme or transporter".

Curation decisions

• DELLA protein-binding IPIs from focused studies: MODIFY -> GO:1990756 ubiquitin-like ligase-substrate adaptor activity. Other protein-binding rows (ASK2, GID1A/B, HT screens): REMOVE per policy.
• regulation of GA signaling (IEA InterPro): MODIFY -> GO:0009939 positive regulation.
• cytoplasm IBA: KEEP_AS_NON_CORE (experimentally nuclear).
• Core: GO:1990756; GA signaling (GO:0009740) + SCF-dependent proteasomal catabolism (GO:0031146); nucleus; SCF complex.