Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Combined Automated Annotation using Multiple IEA Methods
The tryptophan oxygenase gene of Anopheles gambiae.
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The A. gambiae TDO gene was cloned and maps to chromosome 2R
"The Anopheles gambiae gene encoding tryptophan oxygenase, a homolog of the Drosophila melanogaster vermilion gene, has been molecularly cloned and characterized"
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TDO encodes a 392 amino acid protein with 74% identity to Drosophila
"Amino acid comparisons between the deduced protein and previously known tryptophan oxygenases revealed 74% identity between Anopheles and Drosophila"
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TDO transcript is developmentally regulated
"Northern analysis detected a developmentally regulated transcript about 2 kb in length"
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TDO controls eye color and can serve as a transformation marker
"Since this gene is known to control adult eye color in other flies, its cloning from A. gambiae provides the basis for a dominant phenotypic marker for germline transformation"
The Anopheles gambiae tryptophan oxygenase gene expressed from a baculovirus promoter complements Drosophila melanogaster vermilion.
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A. gambiae TDO functionally complements Drosophila vermilion eye color
"The chimeric construct, expressed transiently in vermilion (tryptophan oxygenase) mutants of Drosophila melanogaster, partially rescued adult eye color"
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Demonstrates proper function of the A. gambiae TDO product
"The successful genetic complementation by this construct demonstrated both the proper function of the tryptophan oxygenase product and the effectiveness of the ie-1 promoter in directing expression of foreign genes in live insects"
Patterns of DNA sequence variation in chromosomally recognized taxa of Anopheles gambiae: evidence from rDNA and single-copy loci.
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TDO locus inside inversion 2Rb shows introgressive hybridization between species
"At the tryptophan oxygenase locus inside inversion 2Rb, variation was structured only by inversion orientation and not by taxonomic designation even between An. gambiae and An. arabiensis"
The genome sequence of the malaria mosquito Anopheles gambiae.
Purification and biochemical characterization of a recombinant Anopheles gambiae tryptophan 2,3-dioxygenase expressed in Escherichia coli.
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TDO is the only enzyme initiating tryptophan degradation via the kynurenine pathway in A. gambiae
"In the malaria vector Anopheles gambiae, tryptophan 2,3-dioxygenase (TDO) is the only enzyme able to initiate l-tryptophan degradation through the kynurenine pathway"
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TDO mRNA is ubiquitously expressed across tissues
"a RT-PCR-based analysis of the tissue distribution of TDO mRNA in A. gambiae that revealed a ubiquitous expression of the gene"
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Recombinant TDO purified in heme-ferric form with Michaelis-Menten kinetics determined
"Spectral analyses showed that the enzyme was purified in its heme-ferric form that was subsequently used to determining the Michaelis-Menten constants"
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Several kynurenines and tryptophan metabolites modulate TDO activity
"The screening of a number of compounds as potential TDO modulators showed that several kynurenines and other Tryptophan-derived molecules interfere with the enzyme activity in vitro"
Beyond the eye - Kynurenine pathway impairment causes midgut homeostasis dysfunction and survival and reproductive costs in blood-feeding mosquitoes.
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Kynurenine pathway disruption impairs midgut homeostasis and reproduction in mosquitoes
"Female mosquitoes with spontaneous and induced mutations in the orthologs of the genes encoding kynurenine hydroxylase in Aedes aegypti, Anopheles stephensi and Culex quinquefasciatus exhibited impaired survival and reproductive phenotypes that varied in type and severity among the species"
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Xanthurenic acid (KP end-product) rescues lethality from pathway disruption
"Supplemental xanthurenic acid, a KP end-product, rescued lethality and limited microbiota proliferation in Ae. aegypti"
Anopheline mosquitoes are protected against parasite infection by tryptophan catabolism in gut microbiota.
Crystal structure of the Anopheles gambiae 3-hydroxykynurenine transaminase.
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Xanthurenic acid plays a key role in Plasmodium gametogenesis in A. gambiae
"In Anopheles gambiae, the vector for the most deadly malaria parasite Plasmodium falciparum, xanthurenic acid (XA) plays a key role in parasite gametogenesis and fertility"
Both mosquito-derived xanthurenic acid and a host blood-derived factor regulate gametogenesis of Plasmodium in the midgut of the mosquito.
BioReason-Pro SFT prediction for A. gambiae TDO