Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features
Combined Automated Annotation using Multiple IEA Methods
UniProtKB entry Q88FJ7 (UvrC, Pseudomonas putida KT2440)
-
The reviewed HAMAP-rule record assigns UvrC dual-incision activity and interaction with UvrB.
"UvrC both incises the 5' and 3' sides of the lesion. The N-terminal half is responsible for the 3' incision and the C-terminal half is responsible for the 5' incision."
UvrABC system protein C (Escherichia coli K-12)
Structural and functional insights into the activation of the dual incision activity of UvrC, a key player in bacterial NER
-
Full-length UvrC is the dual-incision endonuclease of bacterial NER; its GIY-YIG (N-terminal) and RNase H-like (C-terminal) nuclease domains perform the 3' and 5' incisions respectively, and UvrC must transition from a closed inactive to an open active state to execute dual incision.
"DNA damage removal is performed by UvrC, an enzyme possessing a dual endonuclease activity, capable of incising the DNA on either side of the damaged site to release a short single-stranded DNA fragment containing the lesion."
A Peek Inside the Machines of Bacterial Nucleotide Excision Repair.
-
Review of bacterial NER describing UvrC recruitment to the UvrB pre-incision complex, its two nuclease domains and its C-terminal (HhH)2 DNA-binding domain.
"UvrC is a dual endonuclease recruited to the UvrB•DNA pre-incision complex to introduce incisions on either side of the lesion [23]."
Catalytic sites for 3' and 5' incision of Escherichia coli nucleotide excision repair are both located in UvrC.
Active site of (A)BC excinuclease. I. Evidence for 5' incision by UvrC through a catalytic site involving Asp399, Asp438, Asp466, and His538 residues.
Regulation of the Escherichia coli excision repair gene uvrC. Overlap between the uvrC structural gene and the region coding for a 24 kD protein.
Cho, a second endonuclease involved in Escherichia coli nucleotide excision repair.
UvrC catalytic-residue conservation versus E. coli UvrC
uvrC review notes (P. putida KT2440)
Falcon deep research report for P. putida uvrC