Identification of an HptB-mediated multi-step phosphorelay in Pseudomonas aeruginosa PAO1.
-
PA1611 signals through HptB to the response regulator PA3346.
"We herein demonstrate that the hybrid sensor PA1611 carries out specific signal transduction, through HptB (PA3345), to the response regulator PA3346 in Pseudomonas aeruginosa PAO1."
Characterization of the histidine-containing phosphotransfer protein B-mediated multistep phosphorelay system in Pseudomonas aeruginosa PAO1.
-
Four sensor kinases transfer phosphoryl groups to HptB in vitro.
"Among the 12 recombinant orphan sensor kinases tested, 4 of these sensors (PA1611, PA1976, PA2824, and RetS) transferred the phosphoryl group to HptB (PA3345)."
-
HptB interacts with each of these sensors in a bacterial two-hybrid assay.
"The in vivo interaction between HptB and each of the sensors was also confirmed using the bacterial two-hybrid assay."
Regulatory RNAs and the HptB/RetS signalling pathways fine-tune Pseudomonas aeruginosa pathogenesis.
-
An hptB deletion is a hyperbiofilm former, complemented by hptB in trans.
"Introduction of the hptB gene cloned in the pUCP18 plasmid (pUCPhptB), into the hptB mutant (PAKΔhptB), resulted in restoration of biofilm and polysaccharide production"
Histidine-containing phosphotransfer protein-B (HptB) regulates swarming motility through partner-switching system in Pseudomonas aeruginosa PAO1 strain.
-
HptB relays phosphoryl groups from multiple sensors to PA3346.
"The histidine-containing phosphotransfer protein-B (HptB; PA3345) is an intermediate protein involved in transferring a phosphoryl group from multiple sensor kinases to the response regulator PA3346 in Pseudomonas aeruginosa PAO1."
The Diguanylate Cyclase HsbD Intersects with the HptB Regulatory Cascade to Control Pseudomonas aeruginosa Biofilm and Motility.
-
An hptB mutant makes more exopolysaccharide, attaches more, twitches more, and swims and swarms less.
"When compared to wild type, the hptB mutant produces more exopolysaccharides [17], attaches more easily to surface, increases twitching motility while it decreases swimming and swarming movements (Figs 2 and 4–6)."
Connected partner-switches control the life style of Pseudomonas aeruginosa through RpoS regulation.
-
RpoS acts downstream of HptB and is the target of the HsbR-HsbA partner switch.
"Altogether, these results indicate that RpoS acts downstream of HtpB in the HtpB cascade and that RpoS is the target of the HsbR/HsbA partner-switch."
Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Electronic Gene Ontology annotations created by ARBA machine learning models