NO-induced biofilm dispersion in Pseudomonas aeruginosa is mediated by an MHYT domain-coupled phosphodiesterase.
-
Recombinant NbdA lacking the MHYT domain is an active PDE.
"Biochemical analyses of recombinant protein variants lacking the membrane-anchored MHYT domain support NbdA being an active PDE"
-
nbdA is required specifically for NO-induced dispersion.
"inactivation of nbdA only impaired biofilm dispersion upon exposure to NO"
-
NO raises NbdA-dependent PDE activity and lowers c-di-GMP in biofilms.
"increased PDE activity, resulting in decreased c-di-GMP levels in biofilms expressing nbdA upon exposure to NO, a response that was absent in the ΔnbdA strain"
Regulation of flagellar motor switching by c-di-GMP phosphodiesterases in Pseudomonas aeruginosa.
-
NbdA inhibits flagellar motor switching through MapZ.
"three of them (DipA, NbdA, and RbdA) can significantly inhibit flagellar motor switching and swimming directional reversal in a MapZ-dependent manner"
Analysis of Pseudomonas aeruginosa diguanylate cyclases and phosphodiesterases reveals a role for bis-(3'-5')-cyclic-GMP in virulence.
-
A PA3311 insertion mutant formed hyperbiofilm (pellicle) and had intermediate virulence; no lysate PDE activity was detected for PA3311.
"A set of mutants in selected DGC- and PDE-encoding genes exhibited attenuated virulence in a mouse infection model."
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt.
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods