The fixed database records support ELAC2 as a precursor-tRNA 3′-processing endoribonuclease. The review keeps a single catalytic core across nuclear and mitochondrial contexts. The four natural splice products are Q9BQ52-1/2/3/4 at 826/501/454/786 residues. Sequence reconstruction does not establish equal activity or localization of these products. Alternative initiation, the annotated processed chain and engineered assay truncations remain distinct.
The PAINT family PTHR12553 contains human target PTN002488759 beneath PTN000284963. Its canonical MSA sequence exactly matches the official 826-residue product. The three annotation sources join literal IBD records at lines 37796, 42928 and 47155 for the catalytic activity, mitochondrial processing and mitochondrial location. The full saved IBD file is scanned along the target path; no target-path IRD or NOT record is present. The donor lists agree exactly with the inherited source objects and current experimental donor records are retained. Neither a small donor set nor target self-evidence is treated as a weakness. The alignment does not functionally resolve the other human splice products.
All nine Reactome source rows resolve through an explicit catalyst to Q9BQ52. Nuclear processing is modeled in nucleoplasm; the heavy- and light-strand reactions place ELAC2 in mitochondrial matrix. ELAC2 performs the 3′ cleavage; mitochondrial RNase P performs the separate 5′ cleavage. The shared 17–826 entity coordinates are database processed-chain metadata rather than authenticated boundaries for every experimental or natural product.
R-HSA-9708296 is tRNA-derived small RNA biogenesis. Its ELAC2 child R-HSA-9708812 removes a pre-tRNA 3′ trailer; it does not demonstrate degradation of mature tRNA. The accepted correction from GO:0016078 to GO:0042780 is lateral, not an ontology descendant refinement. The child reaction's cytosolic entity is a real model, while the original PMID:19933153 experiment fractionates RNA rather than directly localizing ELAC2 protein. The distinct cytosol assertion remains unresolved.
The official HPA XML supports a nucleoplasmic pool and supplies antigen, cell-line and validation metadata. Image pixels were not inspected in this analysis, and the record is not splice-product resolved. ARBA00086796 has target-matching premises for the nucleoid mapping. IPR047151 and EC 3.1.26.11 map to the specific processing activity; IPR027794 supplies a broader tRNA-processing domain mapping. IPR047151 also contains an unrelated PMID:2159360 descriptive citation, retained as an upstream metadata defect without a guessed correction. That defect does not negate the independently established target enzyme activity. Mouse ENSMUSP00000098610.3 maps to Q80Y81-2, not to a human splice product.
All 16 source-term definitions are current and non-obsolete in the fixed retrieval. GO:0042781 is an is_a descendant of both RNA endonuclease activity and tRNA-specific ribonuclease activity. GO:1990180 is a descendant of general tRNA 3′-end processing; the core retains the general process once across both compartments. The mitochondrial nucleoid has an explicit part_of relationship to mitochondrial matrix. These typed relations are kept distinct from experimental localization resolution.
These results replay official data and source identity. They do not replace the original human catalytic/localization experiments or the independent human structural consultation. The two unresolved RNA-capture source measurements, missing original Methods and assay-preparation limits remain explicit in the source-evidence guide.