Gene Ontology annotation through association of InterPro records with GO terms
Automatic assignment of GO terms using logical inference, based on on inter-ontology links
TreeGrafter-generated GO annotations
Combined Automated Annotation using Multiple IEA Methods
UniProtKB record A0A0H2M558 (A0A0H2M558_VARPD) - Cadmium, cobalt and zinc/H(+)-K(+) antiporter from Variovorax paradoxus TBEA6
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VPARA_15480 (czcD, A0A0H2M558) is a 320-aa Cation Diffusion Facilitator (CDF) family transporter (NCBIfam TIGR01297, Pfam PF01545, residues 29-242) with six transmembrane helices and a basic/acidic histidine-rich cytoplasmic loop (residues 155-177).
"FT DOMAIN 29..242
FT /note="Cation efflux protein transmembrane"
FT /evidence="ECO:0000259|Pfam:PF01545""
CzcD is a heavy metal ion transporter involved in regulation of heavy metal resistance in Ralstonia sp. strain CH34.
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In Ralstonia (Cupriavidus) metallidurans CH34, CzcD is a membrane-bound CDF-family transporter that exports cobalt, zinc, and cadmium cations from the cytoplasm, reducing intracellular cation accumulation and regulating czcCBA expression.
"All three proteins mediated a small but significant resistance to cobalt, zinc, and cadmium in Ralstonia, and this resistance was based on a reduced accumulation of the cations. Thus, CzcD appeared to repress the Czc system by an export of the inducing cations."
Responses of Pseudomonas putida to Zinc Excess Determined at the Proteome Level: Pathways Dependent and Independent of ColRS.
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Whole-cell proteomics in Pseudomonas putida showed that the CDF transporter CzcD and the RND efflux pumps CzcCBA1 and CzcCBA2 are induced in response to excess zinc to maintain zinc homeostasis.
"We identified dozens of proteins that responded to zinc in a ColR-independent manner, among others, known metal efflux systems CzcCBA1, CzcCBA2, CadA2 and CzcD."