Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotation based on curation of immunofluorescence data
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Combined Automated Annotation using Multiple IEA Methods
Reconstitution of human Arp2/3 complex reveals critical roles of individual subunits in complex structure and activity.
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The human complex can be reconstituted and p41 contributes to nucleation and stimulation.
"p41,
p21, and p16 contribute differently to nucleation and stimulation by ActA and
WASP"
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The abstract places the prominent filament-binding role in p34/p20 rather than demonstrating isolated p41 affinity.
"p34/p20 bind actin filaments and likely function in actin
branching."
MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Analysis of the myosin-II-responsive focal adhesion proteome reveals a role for β-Pix in negative regulation of focal adhesion maturation.
The Legionella Kinase LegK2 Targets the ARP2/3 Complex To Inhibit Actin Nucleation on Phagosomes and Allow Bacterial Evasion of the Late Endocytic Pathway.
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LegK2 phosphorylates ARPC1B and ARP3; its binding assays and partner-class refinement are separately documented from the original body in the notes.
"LegK2 phosphorylates the ARPC1B and ARP3 subunits
of the ARP2/3 complex."
Isoform diversity in the Arp2/3 complex determines actin filament dynamics.
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ARPC1B/ARPC5L-containing complexes assemble actin more efficiently than the compared ARPC1A/ARPC5 complexes.
"Arp2/3 complexes containing ARPC1B and
ARPC5L are significantly better at promoting actin assembly than those with
ARPC1A and ARPC5, both in cells and in vitro."
Nuclear ARP2/3 drives DNA break clustering for homology-directed repair.
The human Arp2/3 complex is composed of evolutionarily conserved subunits and is localized to cellular regions of dynamic actin filament assembly.
Extension of pseudopodia by myosin-X in a PI3K dependent manner
(WASPs, WAVE):G-actin:ARP2/3 binds F-actin
Branching and elongation of mother and daughter filaments
Role of myosins in phagosome formation
N-WASP binds ARP2/3 and G-actin
IgG:Leishmania surface:FCGR3A translocates from plasma membrane to the parasitophorous vacuole
ARPC1B literature assessment and source-read boundaries
ARPC1B binds WASP to control actin polymerization and curtail tonic signaling in B cells.
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Human patient-derived lymphoblastoid cells have cortical actin defects, and ARPC1B-containing complexes localize to the cortex.
"We found that ARPC1B complexes localized to the actin cortex of control LCL B cell lines and that lines derived from ARPC1B-deficient patients showed decreased and irregular cortical F-actin intensity (Figure 3A)."
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Human Ramos knockout cells corroborate ARPC1B-dependent lamellipodia.
"Like LCLs, control Ramos cells also spontaneously formed lamellipodia in suspension, and these structures were entirely dependent on ARPC1B (Figure 1F)."
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The VCA polymerization experiment uses cell lysates and therefore does not measure isolated ARPC1B nucleation.
"we opted to first grossly determine the maximum contribution of VCA-stimulated ARP2/3 to the overall actin polymerization by performing pyrene-actin polymerization assays in the presence of B cell lysates derived from control or patient cells."
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WASP preferentially coimmunoprecipitates with ARPC1B relative to ARPC1A in the tested cellular assay; this is not a purified binary affinity determination.
"We found that WASP coimmunoprecipitated to a greater extent with ARPC1B as compared with ARPC1A with remarkable consistency (Figure 2, D and F)"
Estrogen and androgen regulate actin-remodeling and endocytosis-related genes during rat spermiation.
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Rat seminiferous-tubule Arpc1b transcripts respond to estrogen through ER-beta; this does not demonstrate the same human tissue response.
"We also find that the
estrogen regulation of Arpc1b and Evl is mediated through estrogen receptor β"
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The rat study localizes ARPC1B and other proteins at or near tubulobulbar complexes.
"Localization of
these proteins at or in the vicinity of tubulobulbar complexes reveals that
ARPC1B, EVL, PICALM, EEA1 and STX5A seem to be involved in spermiation."
Actin related protein complex subunit 1b controls sperm release, barrier integrity and cell division during adult rat spermatogenesis.
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Rat Arpc1b siRNA disrupts tubulobulbar-complex formation and spermiation.
"Here, knockdown of Arpc1b by siRNA in adult rat
testis led to defects in spermiation caused by failure in TBC formation."
Loss of the Arp2/3 complex component ARPC1B causes platelet abnormalities and predisposes to inflammatory disease.
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Human ARPC1B is prominent in blood cells, consistent with enrichment rather than exclusive expression.
"Human actin-related protein 2/3 complex (Arp2/3), required for actin filament
branching, has two ARPC1 component isoforms, with ARPC1B prominently expressed
in blood cells."
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ARPC1B predominates in normal human platelets; isoform ratios vary among tissues.
"We observed low expression of ARPC1A relative to ARPC1B in normal platelets, and increased ARPC1A in ARPC1B-deficient cells (Fig. 2b,c)."
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Human ARPC1B deficiency associates platelet abnormalities with hematopoietic and immune manifestations.
"Thus loss of
ARPC1B produces a unique set of platelet abnormalities, and is associated with
haematopoietic/immune symptoms affecting cell lineages where this isoform
predominates."