Gene Ontology annotation through association of InterPro records with GO terms.

InterPro2GO supplies family/domain-based automated annotations that require curator review against the specific PP_4519/LapE protein context.

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

UniProt subcellular-location mapping provides automated cellular-component annotations based on reviewed-entry localization vocabulary.

GO_REF:0000118

TreeGrafter-generated GO annotations

TreeGrafter transfers GO terms from phylogenetic families; curator review checks whether the transferred term matches the P. putida ortholog.

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods.

The combined UniProt automated pipeline annotation provides IEA terms that must be checked for specificity and evidence fit.

PMID:12534463

Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440.

KT2440 genome sequencing establishes PP_4519 in the strain background under review.

PMID:35682576

Pseudomonas putida Biofilm Depends on the vWFa-Domain of LapA in Peptides-Containing Growth Medium.

LapA biofilm work supports the Lap secretion context used to interpret PP_4519/tolC as the LapE outer-membrane exit duct.

file:PSEPK/tolC/tolC-uniprot.txt

UniProt entry Q88EE6

UniProt places Q88EE6 in the cell outer membrane and identifies it as an outer membrane factor family protein.

file:PSEPK/tolC/tolC-notes.md

Curator notes on PP_4519/tolC/LapE identity and function

PP_4519 is a TolC-family outer membrane factor better interpreted as LapE in KT2440.

"In the P. putida KT2440 LapA biofilm literature, PP_4519 corresponds to `lapE`, the outer membrane component of the Lap secretion apparatus rather than a generic standalone multidrug efflux pump subunit"
LapE is required for LapA externalization.

"The same study explicitly describes LapE as the third part of the LapA transporter and states that loss of lapE blocks LapA export to the cell surface and weakens biofilm formation"
Generic efflux GO terms likely overgeneralize the family-level TolC assignment.

"These data support treating PP_4519/tolC as a TolC-like outer membrane exit duct reused in a type I protein secretion system for LapA surface display."

file:PSEPK/tolC/tolC-deep-research-falcon.md

Falcon deep research on PP_4519/tolC in Pseudomonas putida KT2440

PP_4519 is a TolC-like outer membrane factor for tripartite efflux or secretion assemblies.

"**PP_4519 (tolC; Q88EE6) encodes a TolC-like outer membrane factor that likely serves as an outer-membrane exit duct for one or more tripartite efflux/secretion assemblies.**"
Transporter pairing and substrate scope remain incompletely mapped for PP_4519 in KT2440.

"Direct evidence mapping **PP_4519/Q88EE6** to a specific KT2440 tripartite pump (inner-membrane transporter + adaptor) and to a defined set of substrates was not found in the retrieved full-text evidence"