Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniPathway vocabulary mapping
Gene Ontology annotation based on curation of immunofluorescence data
Combined Automated Annotation using Multiple IEA Methods
Identification of the peroxisomal beta-oxidation enzymes involved in the biosynthesis of docosahexaenoic acid.
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The peroxisomal beta-oxidation of C24:6n-3 to C22:6n-3 (DHA) is catalyzed by SCOX (acyl-CoA oxidase), DBP (D-bifunctional protein), and both 3-ketoacyl-CoA thiolase (ACAA1) and SCPx, implicating ACAA1 in peroxisomal PUFA chain shortening.
"SCOX, DBP, and both 3-ketoacyl-CoA thiolase and SCPx. These findings are of"
Subcellular localisation and processing of non-specific lipid transfer protein are not aberrant in Rhizomelic Chondrodysplasia Punctata fibroblasts.
Peroxisomes in human and mouse testis: differential expression of peroxisomal proteins in germ cells and distinct somatic cell types of the testis.
Contribution of peroxisome-specific isoform of Lon protease in sorting PTS1 proteins to peroxisomes.
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pLon (peroxisomal Lon/LONP2) does NOT process the PTS2-containing 3-ketoacyl-CoA thiolase, indicating no functional pLon-mediated processing of ACAA1.
"does not process PTS2-containing"
Pex3p-dependent peroxisomal biogenesis initiates in the endoplasmic reticulum of human fibroblasts.
Defining the membrane proteome of NK cells.
Structural requirements for interaction of peroxisomal targeting signal 2 and its receptor PEX7.
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ACAA1 (acyl-CoA thiolase) is a mammalian PTS2-carrying peroxisomal matrix protein that exerts the last step of fatty acid beta-oxidation and is imported via interaction of its N-terminal PTS2 with PEX7.
"acyl-CoA thiolase exerting the last step of fatty acid"
Bile acid profiles in peroxisomal 3-oxoacyl-coenzyme A thiolase deficiency.
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A patient whose liver contained no immunoreactive peroxisomal 3-oxoacyl-CoA thiolase accumulated C27 bile-acid intermediates (varanic acid, THCA), implicating ACAA1 in VLCFA/cholestanoic acid metabolism.
"liver contained no immunoreactive peroxisomal 3-oxoacyl-CoA thiolase"
Rhizomelic chondrodysplasia punctata. Deficiency of 3-oxoacyl-coenzyme A thiolase in peroxisomes and impaired processing of the enzyme.
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Reduced peroxisomal 3-oxoacyl-CoA thiolase activity decreases the rate of peroxisomal beta-oxidation of palmitoyl-CoA, and thiolase is detected in catalase-containing (peroxisomal) fractions.
"results in a decrease in the rate of peroxisomal"
Mechanistic insights into PTS2-mediated peroxisomal protein import: the co-receptor PEX5L drastically increases the interaction strength between the cargo protein and the receptor PEX7.
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Thiolase is used as the prototypical PTS2 cargo whose import into the peroxisomal matrix requires a trimeric PEX7-PEX5L-cargo complex.
"PTS2-carrying proteins interact with their cognate receptor protein PEX7"
Human peroxisomal 3-oxoacyl-coenzyme A thiolase deficiency.
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Deficiency of human peroxisomal 3-oxoacyl-CoA thiolase (acyl-CoA:acetyl-CoA C-acyltransferase, EC 2.3.1.16) causes very low peroxisomal beta-oxidation activity and accumulation of very-long-chain fatty acids and bile-acid intermediates; adding purified thiolase restored beta-oxidation.
"the deficiency of peroxisomal 3-oxoacyl-CoA thiolase is responsible for the very low peroxisomal beta-oxidation activity"
Immunocytochemical demonstration of peroxisomal enzymes in human kidney biopsies.
A reference map of the human binary protein interactome.
Immunocytochemical localization of peroxisomal proteins in human liver and kidney.
alpha-linolenic acid (ALA) metabolism
Formation of DHA-CoA catalysed by 3-ketoacyl-CoA thiolase
Beta-oxidation of very long chain fatty acids
3-ketohexacosanoyl-CoA + CoASH => tetracosanoyl-CoA + acetyl-CoA
Exocytosis of specific granule lumen proteins
PEX7 binds cargo proteins containing PTS2
Cargo of PEX5L:PEX7 translocates from the cytosol to the peroxisomal matrix