Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Lateral relocation of auxin efflux regulator PIN3 mediates tropism in Arabidopsis.
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Native PIN3 localization and gravity-dependent relocation link transport to tropism.
"In the root columella, PIN3 is positioned symmetrically at the plasma membrane but rapidly relocalizes laterally on gravity stimulation."
Brassinosteroids stimulate plant tropisms through modulation of polar auxin transport in Brassica and Arabidopsis.
PINOID positively regulates auxin efflux in Arabidopsis root hair cells and tobacco cells.
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PIN3 overexpression increases auxin efflux and inhibits hair growth in the tested systems.
"In an auxin efflux assay using transgenic tobacco suspension cells, PIDox or PIN3ox also enhanced auxin efflux."
Differential auxin-transporting activities of PIN-FORMED proteins in Arabidopsis root hair cells.
Light-mediated polarization of the PIN3 auxin transporter for the phototropic response in Arabidopsis.
Functional identification of the phosphorylation sites of Arabidopsis PIN-FORMED3 for its subcellular localization and biological role.
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PIN3 phosphosite mutations alter trafficking and root growth/gravitropism.
"Mutations of these phosphorylatable residues disrupted in planta phosphorylation of PIN3 and its subcellular trafficking, and caused defects in PIN3-mediated biological processes such as auxin efflux activity, auxin maxima formation, root growth, and root gravitropism."
Global Analysis of Membrane-associated Protein Oligomerization Using Protein Correlation Profiling.
Structures and mechanisms of the Arabidopsis auxin transporter PIN3.
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PIN3 structures resolve a homodimer with IAA/NPA recognition.
"A. thaliana PIN3 exists as a homodimer, and its transmembrane helices 1, 2 and 7 in the scaffold domain are involved in dimerization."
Alkaline stress reduces root waving by regulating PIN7 vacuolar transport.
Biosynthesis- and transport-mediated dynamic auxin distribution during seed development controls seed size in Arabidopsis.
Structures and mechanisms of the Arabidopsis auxin transporter PIN3
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Publisher extended-data captions include PIN3 radiolabeled efflux and dimer-interface assays.
"The 3H-IAA efflux of WT and mutant PIN3 at 10 min in the IAA loading phase in the absence or presence of 50 µM NPA"
Target-specific inspection of PMID28887381 supplemental proteomics
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Inspection of all original supplemental workbooks resolves the target identification and experimental classification.
"PIN3 AT1G70940.1 appears in S1 row 1879 with the same membrane/blank-dual/PM summary."
Sequential induction of auxin efflux and influx carriers regulates lateral root emergence.
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Native localization and time-course experiments, combined with a multicellular model, support sequential cortical PIN3 then LAX3 induction during lateral-root emergence.
"we found that auxin induction of LAX3 expression was slower when compared with PIN3"