LEU2 notes (YCL018W, P04173)

• 3-isopropylmalate dehydrogenase, EC 1.1.1.85, NAD+-dependent, step 3 of Leu branch [UniProt:P04173].
• Genetic/biochemical: PMID:5724969
• Cytosolic [PMID:4355481, soluble fraction with G6PDH marker].
• Promiscuous activity on beta-ethylmalate PMID:23642236

Curation observations

• GO:0006097 glyoxylate cycle (IMP, PMID:23642236) REMOVED: full text read; the work concerns a proposed glycine/glyoxylate -> butanol route, not the isocitrate lyase/malate synthase cycle.
• PMID:5724969 added as a reference (not in GOA for LEU2) to support the activity.